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Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics

Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics

Published on: June 17, 2012

An open source plant kinase chemogenomics set.

Maria Florencia Ercoli¹, Priscila Zonzini Ramos², Rashmi Jain¹

¹Department of Plant Pathology and the Genome Center University of California Davis CA USA.

|November 30, 2022

Summary

This summary is machine-generated.

Researchers screened rice kinases with human kinase inhibitors, finding compounds that inhibit root growth in both Arabidopsis and rice. This suggests human kinase inhibitors can be valuable tools for studying plant kinase functions.

Keywords:

Oryza sativa compound screening ligation‐independent cloning plant kinases protein production thermal shift assay

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Area of Science:

Plant biology
Biochemistry
Molecular biology

Background:

The kinome, the complete set of protein kinases, plays crucial roles in cellular signaling pathways in all organisms.
Understanding plant kinomes is essential for agricultural advancements and crop improvement.

Purpose of the Study:

To explore the functional conservation between human and plant kinases.
To identify novel chemical probes for dissecting plant kinase functions, particularly in root development.

Main Methods:

Cloning and expression of 129 rice (Oryza sativa) protein kinases in Escherichia coli.
Purification and screening of 40 rice kinases against 627 diverse human kinase inhibitors using differential scanning fluorimetry (DSF).
Testing of active compounds for effects on primary root development in Arabidopsis and identifying target plant kinases.

Main Results:

14 out of 37 active compounds significantly reduced primary root length in Arabidopsis.
Two compounds inhibited root growth by binding to the predicted orthologue of Arabidopsis PSKR1, a receptor for the peptide hormone PSK.
Six compounds that inhibited Arabidopsis root growth also affected rice root growth, and RAF265 bound to nine conserved rice kinases.

Conclusions:

Human kinase inhibitor libraries are effective tools for identifying inhibitors of plant kinases.
The identified compounds can be used to investigate the roles of plant kinases, including those involved in root development.
Functional conservation exists between human and plant kinases, enabling cross-species application of chemical probes.