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Related Experiment Video

Updated: Aug 19, 2025

Cerenkov Luminescence Imaging CLI for Cancer Therapy Monitoring
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Monitor Tumor pHe and Response Longitudinally during Treatment Using CEST MRI-Detectable Alginate Microbeads.

Peng Xiao1, Jianpan Huang1, Xiongqi Han1

  • 1Department of Biomedical Engineering, City University of Hong Kong, Hong Kong, China.

ACS Applied Materials & Interfaces
|November 30, 2022
PubMed
Summary

New pH-sensing microbeads enable longitudinal imaging of tumor microenvironment pH changes during treatment. These Iop-lipobeads offer a sensitive, noninvasive method for monitoring therapeutic responses using chemical exchange saturation transfer (CEST) contrast.

Keywords:
CESTIopamidolbicarbonate treatmentmicrofluidicspH monitor

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Medical Imaging

Background:

  • Imaging the tumor microenvironment's pH (pHe) is crucial for understanding tumor aggressiveness and treatment efficacy.
  • Current methods for monitoring pHe longitudinally and during therapy are limited.

Purpose of the Study:

  • To develop and validate a novel pH-sensing microbead for noninvasive, longitudinal imaging of tumor microenvironment pHe changes.
  • To assess the microbeads' performance during sodium bicarbonate treatment.

Main Methods:

  • pH-sensing microbeads (Iop-lipobeads) were fabricated using clinically approved materials (alginate, liposomes with iopamidol) via microfluidics.
  • Chemical Exchange Saturation Transfer (CEST) imaging was used to quantify the contrast generated by Iop-lipobeads.
  • In vitro and in vivo studies were conducted to evaluate stability, pH sensitivity, and performance during treatment.

Main Results:

  • Iop-lipobeads generated significant CEST contrast (10.6% at pH 7.0) with good in vitro stability.
  • CEST contrast decreased with decreasing pH, demonstrating pH sensitivity.
  • In vivo, Iop-lipobeads showed increased CEST contrast during sodium bicarbonate treatment (19.7 ± 6.1%), exceeding amide CEST changes.
  • Contrast decreased after treatment cessation (15.2 ± 4.8%).

Conclusions:

  • Iop-lipobeads provide a robust and sensitive method for imaging tumor microenvironment pHe.
  • This approach allows for noninvasive, longitudinal monitoring of therapeutic effects.
  • Iop-lipobeads offer an independent assessment of pHe changes, complementing existing CEST techniques.