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Updated: Aug 19, 2025
A Murine Model of Group B Streptococcus Vaginal Colonization
Published on: November 16, 2016
Performance of the HiberGene Group B Streptococcus kit, a loop-mediated isothermal amplification-based assay for GBS
Mireille Tittel-Elmer1, Begoña Martinez de Tejada2,3, Gesuele Renzi4
1Bacteriology Laboratory, Division of Laboratory Medicine, Department of Diagnostics, Geneva University Hospitals, Geneva, Switzerland. Mireille.Tittel-Elmer@hcuge.ch.
Abstract:
Timely and accurate detection of Group B Streptococcus (GBS) carriage in pregnant women allows for targeted peripartum prophylaxis. Replacing culture-based screening by molecular biology assays enables faster results obtention, better targeted antibiotic prophylaxis, and reduces the laboratory workload. Here, we present a comparative analysis between a Loop Mediated Isothermal Amplification assay (HiberGene GBS kit) and culture (gold-standard). The HiberGene GBS kit showed a sensitivity of 97.9% and a specificity of 96.8% compared with culture. The limit of detection was estimated at 103 cfu/ml and results were obtained within 30 min. HiberGene GBS assay can be used for peripartum GBS screening and targeted antibiotic prophylaxis provided sample processing can be swiftly performed around the clock.
