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Updated: Aug 19, 2025

Methodology for Sputum Induction and Laboratory Processing
Published on: December 17, 2017
Development and validity of an educational folder for pulmonary tuberculosis sputum collection
Karine Nascimento da Silva1, Sabrina Alaide Amorim Alves1, Maria do Socorro Vieira Lopes1
1Universidade Regional do Cariri. Crato, Ceará, Brazil.
This study developed and validated an educational folder for pulmonary tuberculosis (TB) sputum collection. The validated material is suitable for improving correct sputum collection and enabling accurate TB diagnosis.
Area of Science:
- Medical Education
- Infectious Diseases
- Diagnostic Tools
Background:
- Pulmonary tuberculosis (TB) diagnosis relies heavily on sputum collection quality.
- Effective patient education is crucial for obtaining high-quality sputum samples.
- Existing educational materials may lack comprehensive content or user-friendly design.
Purpose of the Study:
- To develop and validate an educational folder for pulmonary tuberculosis sputum collection.
- To ensure the material is accurate, clear, and easy to understand for patients.
- To improve the quality of sputum samples for TB diagnosis.
Main Methods:
- A methodological study involving four phases: project design, literature review, material development, and validity assessment.
- Content and appearance validity were assessed using the Content Validity Index (CVI), Kappa Coefficient, and Suitability Assessment of Materials (SAM).
- Expert judges evaluated the educational folder for accuracy, clarity, and suitability.
Main Results:
- An educational folder detailing recommended steps for quality sputum collection was successfully developed.
- The folder achieved an overall Content Validity Index (CVI) of 0.90, indicating strong expert agreement.
- High inter-rater reliability was demonstrated with a total Kappa of 0.83, and the material showed superior suitability.
Conclusions:
- The developed educational folder is valid, reliable, and suitable for its intended purpose.
- Its simple, attractive language, illustrations, and layout enhance user comprehension and engagement.
- The material has significant potential to improve sputum collection accuracy, thereby aiding correct tuberculosis diagnosis.
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