Related Experiment Video
Updated: Jun 17, 2026
08:17
Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems
Published on: July 4, 2011
15.4K
NIR-II Dyad-Doped Ratiometric Nanosensor with Enhanced Spectral Fidelity in Biological Media for In Vivo Biosensing
Peng Yu1, Kui Yan1, Shangfeng Wang1
1Department of Chemistry, State Key Laboratory of Molecular Engineering of Polymers, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials and iChem, Fudan University, Shanghai 200433, China.
Nano Letters
|December 1, 2022
Summary
We developed a novel ratiometric fluorescence nanosensor for accurate in vivo sensing. This advanced sensor overcomes spectral distortion in biological media, enabling reliable monitoring of oxidative stress.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Analytical Chemistry
Background:
- Ratiometric fluorescence nanosensors offer quantitative biological insights but suffer from spectral shifts in vivo.
- These spectral distortions limit the accuracy and in vivo applicability of current nanosensor technologies.
Purpose of the Study:
- To develop a highly stable ratiometric fluorescence nanosensor for accurate in vivo sensing in the second near-infrared (NIR-II) window.
- To address spectral distortion and improve sensing fidelity in biological environments.
Main Methods:
- Synthesized a fluorescent dyad (aBOP-IR1110) by linking aza-BODIPY with a peroxynitrite (ONOO-) responsive thiocyanine.
- Encapsulated the dyad within PEGylated nanomicelles to enhance spectral stability and hydrophobicity.
- Investigated intramolecular Förster resonance energy transfer (FRET) for ratiometric response to ONOO-.
Main Results:
- The encapsulated dyad demonstrated over 9.4 times improved spectral fidelity in serum compared to noncovalent counterparts.
- Achieved a linear ratiometric response with enhanced serum tolerance due to increased molecular weight and hydrophobicity.
- Successfully monitored oxidative stress dynamics in traumatic brain injury models and evaluated therapeutic efficacy with high correlation to in vitro assays.
Conclusions:
- The developed NIR-II dyad-doped nanosensor exhibits high spectral fidelity and serum tolerance for in vivo applications.
- This technology holds significant potential for high-fidelity, quantitative sensing of biological processes like oxidative stress in vivo.
- The nanomicelle encapsulation strategy effectively shields the sensor from biological interference, enhancing its reliability.
