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Integrated Exposomics/Metabolomics for Rapid Exposure and Effect Analyses.

Mira Flasch1,2, Veronika Fitz2,3, Evelyn Rampler3

  • 1Faculty of Chemistry, Department of Food Chemistry and Toxicology, University of Vienna, Währinger Straße 38-40, 1090 Vienna, Austria.

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Summary

This study introduces a new method to measure both the human metabolome and chemical exposome simultaneously. This rapid, integrated analysis aids in understanding environmental health impacts and disease drivers.

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Area of Science:

  • Environmental Health Sciences
  • Analytical Chemistry
  • Metabolomics

Background:

  • The human exposome, encompassing all environmental exposures, is complex and poorly understood.
  • Current methods for analyzing chemical exposures and metabolic pathways are challenging and time-consuming.
  • Integrated analysis of the metabolome and exposome is crucial for understanding environmental health impacts.

Purpose of the Study:

  • To develop a novel, rapid, and comprehensive analytical approach for simultaneous measurement of the endogenous metabolome and chemical exposome.
  • To establish a high-throughput method for assessing environmental exposures and their link to metabolic disruption.
  • To validate a new technology for analyzing diverse molecules in biological samples.

Main Methods:

  • Development of a dual-column liquid chromatography system combining reverse-phase and HILIC with fast polarity-switching.
  • Integration of effluents before analysis using high-resolution mass spectrometry (LC-HRMS).
  • Benchmarking performance using standard reference materials and authentic standards, including analysis of urine and plasma samples.

Main Results:

  • Achieved highly sensitive median limits of detection (LODs) for over 140 endogenous metabolites and 100 model xenobiotics/estrogens.
  • Demonstrated effective analysis in biological matrices with median LODs of 0.7 ng/mL (urine) and 0.5 ng/mL (plasma) for exogenous chemicals.
  • Successfully applied the method to real-life urine samples from distinct geographical regions (sub-Saharan Africa and Europe).

Conclusions:

  • The novel LC-HRMS approach enables simultaneous, quantitative assessment of the endogenous metabolome and chemical exposome.
  • This technology facilitates high-throughput measurement of environmental drivers of diseases.
  • The method provides a powerful tool for exposome research and environmental health monitoring.