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Related Concept Videos

Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

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Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
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Bacterial Phylum Bacteroidota01:26

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The phylum Bacteroidota includes over 700 species classified into four primary orders: Bacteroidales, Cytophagales, Flavobacteriales, and Sphingobacteriales. These gram-negative, non-sporulating rods exhibit saccharolytic capabilities and can be aerobic or fermentative, encompassing obligate aerobes, facultative aerobes, and obligate anaerobes. Many species display gliding motility, though some are nonmotile or use flagella. The genus Bacteroides is well-studied due to its significant role in...
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Bacteroides fragilis derived metabolites, identified by molecular networking, decrease Salmonella virulence in mice

Thomas Gautier1, Nolwenn Oliviero1, Solenn Ferron2

  • 1INSERM, Univ Rennes, INRAE, UMR 1241, Nutrition Metabolisms and Cancer Institute, Rennes, France.

Frontiers in Microbiology
|December 5, 2022
PubMed
Summary
This summary is machine-generated.

Bacteroides fragilis metabolites inhibit Salmonella Heidelberg translocation and reduce inflammation without altering gut microbiota diversity. These findings suggest potential therapeutic applications for gut health.

Keywords:
Bacteroides fragilischolic acidfractionationmetabolitesmicrobiotamolecular networkingsupernatantvirulence factors

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Area of Science:

  • Microbiology
  • Gut Microbiota
  • Bacterial Pathogenesis

Background:

  • Gut resident bacteria protect against pathogens via metabolite production.
  • Bacteroides fragilis and its supernatant previously inhibited Salmonella Heidelberg translocation in vitro.

Purpose of the Study:

  • Identify bioactive molecules in B. fragilis supernatant responsible for inhibiting Salmonella translocation.
  • Evaluate the efficacy and impact of these bioactive fractions in vivo.

Main Methods:

  • Liquid Chromatography High-Resolution Tandem Mass Spectrometry (LC-HRMS/MS) for molecule identification.
  • In vitro intestinal epithelium model and in vivo BALB/c mouse model.
  • Molecular networking for compound characterization.

Main Results:

  • Two fractions (F3, F4) strongly inhibited S. Heidelberg translocation.
  • Bioactive fractions reduced S. Heidelberg in mice, decreased inflammatory cytokines and neutrophils, and altered Alistipes genus.
  • Cholic acid (CA) identified as an active compound, inhibiting Salmonella virulence genes (sipA).
  • Bioactive fractions downregulated Salmonella flagellar gene (fliC) and did not affect gut microbiota diversity.

Conclusions:

  • B. fragilis metabolites, including cholic acid, effectively inhibit Salmonella virulence and translocation.
  • These findings support the development of B. fragilis-derived metabolites as therapeutics against pathogenic bacteria.
  • Targeting bacterial virulence factors offers a strategy to combat infections without disrupting the gut microbiota.