Flow analysis and hydraulic performance of denitrifying bioreactors under different carbon dosing treatments

Denitrifying bioreactors are an effective approach for removing nitrate from a variety of non-point wastewater sources, including agricultural tile drainage. However, compared to alternate mitigation approaches such as constructed wetlands, nitrate removal in bioreactors may decline with time and low temperature, resulting in poor long-term nitrate removal rates. To address the low nitrate removal rates in bioreactors, the addition of an external carbon source has been found to be an effective method for enhancing and maintaining nitrate removal rates. While carbon dosing has led to a significant improvement in nitrate removal, some of the possible adverse effects of carbon dosing, such as clogging and reduction in hydraulic efficiency, remain unknown and need to be investigated. Using observations from both field and mesocosm trials, we compared the hydraulic performance of bioreactors with and without carbon dosing. The pilot-scale field bioreactor (58 m³ total woodchip volume, 25 m³ saturated volume, referred to as field bioreactor in this work) treated drainage water from a paddock of a dairy farm. The bioreactor received an exogenous carbon dose of 8% methanol (v/v) at 10 mL min^-1 and 5 mL min^-1 in the 2020 and 2021 drainage seasons, respectively. The field bioreactor had a statistically higher hydraulic conductivity in 2018 when not carbon-dosed of 4601 m day^-1, reducing to 1600 m day^-1 in 2021 which was the second year of carbon dosing. Field observations could not establish whether the addition of liquid carbon could affect the bioreactor's internal hydraulics performance, such as actual hydraulic retention time (AHRT), despite a significant decline in hydraulic conductivity in the field bioreactor. Separate experiments on replicated bioreactor mesocosms were conducted to investigate the effects of carbon dosing on the internal hydraulic parameters of bioreactors. These mesocosm bioreactors had previously been used to study the long-term effects of methanol dosing on bioreactor performance, such as nitrate removal under steady-state conditions. The mesocosm and field bioreactors shared some characteristics, such as the use of methanol as an external carbon source, but the mesocosm experiments were hydrologically controlled contrary to the field bioreactor's transient operating conditions. We found that methanol dosing in either carbon or nitrate limiting conditions had no significant effects (p-value >0.05) on internal hydraulic parameters (e.g., effective utilization of media) when compared to control bioreactors. The present study offers insight into the long-term hydraulic performance of bioreactors and may help develop small-footprint bioreactors that incorporate external carbon dosing.