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Related Experiment Video

Updated: Aug 18, 2025

Purification of Platelets from Mouse Blood
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Published on: May 7, 2019

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High-purity isolation platelets by gradient centrifugation plus filtration.

Jia Jin1,2, Yilin Shao3, Jian Zhang2,4

  • 1Department of Lymphoma, Fudan University Shanghai Cancer Center, Shanghai, China.

International Journal of Laboratory Hematology
|December 5, 2022
PubMed
Summary

A new method using gradient centrifugation plus filtration efficiently isolates high-purity platelets from blood samples. This technique, effective within 48 hours without platelet inhibition cocktail, offers a reliable approach for liquid biopsy applications.

Keywords:
flow cytometrynext-generation sequencingplatelet isolationtumor educated platelet

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Hematology

Background:

  • Platelets serve as a valuable liquid biopsy tool for real-time tumor pathology and treatment monitoring.
  • Current methods for isolating pure platelets lack efficiency.

Purpose of the Study:

  • To evaluate and compare three distinct platelet isolation techniques.
  • To identify an efficient and high-purity method for platelet isolation.

Main Methods:

  • Compared gradient centrifugation alone, gradient centrifugation with filtration, and gradient centrifugation with magnetic beads separation.
  • Assessed platelet recovery and purity using flow cytometry (CD61, CD45, CD62P markers) and gene expression analysis (qPCR, NGS).
  • Determined optimal time limits for room temperature platelet isolation.

Main Results:

  • Gradient centrifugation plus filtration yielded superior results with 9.1% recovery and 99.98% purity.
  • Platelet activation levels were unaffected by the use of platelet inhibition cocktail (PIC).
  • Platelet RNA integrity was maintained for up to 48 hours post-collection at room temperature.

Conclusions:

  • Gradient centrifugation plus filtration is a novel, highly efficient protocol for isolating pure platelets.
  • The optimized method requires no PIC and can be performed at room temperature within 48 hours of blood collection.
  • This technique enhances the utility of platelets in liquid biopsy.