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A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
Published on: October 15, 2013
15.0K
Advances in electrochemiluminescence for single-cell analysis.
Qian Yang1,2, Xiaoyu Huang1, Beibei Gao1
1School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China. wangfu@sjtu.edu.cn.
The Analyst
|December 7, 2022
Summary
Single-cell electrochemiluminescence (SCECL) offers high-resolution bioanalysis. This review highlights advances in SCECL for understanding cell heterogeneity and developing new bioimaging and therapeutic tools.
Area of Science:
- Analytical Chemistry
- Biotechnology
- Cell Biology
Background:
- Innovative analytical methods enable single-cell and subcellular analysis.
- Electrochemiluminescence (ECL) offers high sensitivity, a wide dynamic range, and reproducibility.
- Single-cell ECL (SCECL) analysis provides excellent spatiotemporal resolution for bioanalysis.
Purpose of the Study:
- To review recent advances in single-cell electrochemiluminescence (SCECL) analysis and bioimaging.
- To highlight progress in ECL probes and system design for SCECL.
- To discuss the applications of SCECL in bioassays, bioimaging, and therapeutics.
Main Methods:
- Review of historical and recent developments in ECL probes and system design.
- Analysis of representative SCECL techniques for bioassays and bioimaging.
- Exploration of SCECL applications in therapeutic strategies.
Main Results:
- SCECL analysis has emerged as a powerful tool for revealing cellular heterogeneity and stochastic processes.
- Recent advances have improved the sensitivity and spatiotemporal resolution of SCECL.
- SCECL shows promise for advanced bioassays, high-resolution bioimaging, and targeted therapeutics.
Conclusions:
- SCECL is a rapidly advancing field with significant potential in bioanalysis and bioimaging.
- Continued development of ECL probes and systems will enhance SCECL capabilities.
- SCECL offers a promising platform for future bioanalytical, imaging, and therapeutic applications.
