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Related Concept Videos

CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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CRISPR/Cas9 Genome Editing01:28

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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MicroRNAs01:22

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
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The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

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CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
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RNA Interference01:23

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
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Updated: Aug 18, 2025

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
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CRISPR/Cas-Based MicroRNA Biosensors.

Qian Zhang1, Xinyi Zhang2, Xiaoran Zou1

  • 1College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan, 250014, P.R. China.

Chemistry (Weinheim an Der Bergstrasse, Germany)
|December 8, 2022
PubMed
Summary
This summary is machine-generated.

Clustered regularly interspaced short palindromic repeats/CRISPR-associated (CRISPR/Cas) systems offer precise and efficient tools for detecting microRNAs (miRNAs). This review explores recent advancements in CRISPR/Cas-based biosensors for miRNA detection and disease biomarker applications.

Keywords:
CRISPR/Casbiomarkerbiosensordisease diagnosismiRNA

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • MicroRNAs (miRNAs) are crucial post-transcriptional regulators involved in numerous cellular functions.
  • Aberrant miRNA expression is linked to various diseases, including cancers, making them valuable disease biomarkers.
  • The CRISPR/Cas system provides precise targeting and high cleavage activity, ideal for biosensor development.

Purpose of the Study:

  • To review recent advancements in CRISPR/Cas-based biosensors for microRNA detection.
  • To summarize the principles, features, and performance of these novel miRNA detection systems.
  • To identify current challenges and future research directions in the field.

Main Methods:

  • Review of recent scientific literature on CRISPR/Cas systems and miRNA detection.
  • Analysis of the mechanisms and design principles of CRISPR/Cas-based miRNA biosensors.
  • Evaluation of reported performance metrics for various miRNA detection platforms.

Main Results:

  • CRISPR/Cas systems demonstrate high specificity and sensitivity for detecting miRNAs.
  • Various CRISPR/Cas-based biosensor designs have been developed, showcasing diverse applications.
  • These biosensors offer advantages over traditional methods in terms of speed and simplicity.

Conclusions:

  • CRISPR/Cas-based biosensors represent a promising technology for sensitive and specific miRNA detection.
  • Further research is needed to optimize these biosensors for clinical diagnostics and disease monitoring.
  • Addressing current challenges will facilitate the translation of CRISPR/Cas technology into practical applications.