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CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
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CRISPR/Cas-Based MicroRNA Biosensors

Qian Zhang1, Xinyi Zhang2, Xiaoran Zou1

  • 1College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan, 250014, P.R. China.

Chemistry (Weinheim an Der Bergstrasse, Germany)
|December 8, 2022
PubMed

Insights

Clustered regularly interspaced short palindromic repeats/CRISPR-associated (CRISPR/Cas) systems offer precise and efficient tools for detecting microRNAs (miRNAs). This review explores recent advancements in CRISPR/Cas-based biosensors for miRNA detection and disease biomarker applications.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • MicroRNAs (miRNAs) are crucial post-transcriptional regulators involved in numerous cellular functions.
  • Aberrant miRNA expression is linked to various diseases, including cancers, making them valuable disease biomarkers.
  • The CRISPR/Cas system provides precise targeting and high cleavage activity, ideal for biosensor development.

Purpose of the Study:

  • To review recent advancements in CRISPR/Cas-based biosensors for microRNA detection.
  • To summarize the principles, features, and performance of these novel miRNA detection systems.
  • To identify current challenges and future research directions in the field.

Main Methods:

  • Review of recent scientific literature on CRISPR/Cas systems and miRNA detection.
  • Analysis of the mechanisms and design principles of CRISPR/Cas-based miRNA biosensors.
  • Evaluation of reported performance metrics for various miRNA detection platforms.

Main Results:

  • CRISPR/Cas systems demonstrate high specificity and sensitivity for detecting miRNAs.
  • Various CRISPR/Cas-based biosensor designs have been developed, showcasing diverse applications.
  • These biosensors offer advantages over traditional methods in terms of speed and simplicity.

Conclusions:

  • CRISPR/Cas-based biosensors represent a promising technology for sensitive and specific miRNA detection.
  • Further research is needed to optimize these biosensors for clinical diagnostics and disease monitoring.
  • Addressing current challenges will facilitate the translation of CRISPR/Cas technology into practical applications.
Keywords:
CRISPR/Casbiomarkerbiosensordisease diagnosismiRNA

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