Related Experiment Video
Updated: Aug 18, 2025
Enhanced Oil Recovery using a Combination of Biosurfactants
Published on: June 3, 2022
Evaluation of novel surfactants for plant transformation
Jennie Huynh1, Sara K Hotton1, Ron Chan1
1Crop Improvement and Genetics, Western Regional Research Center, USDA-ARS, Albany, CA, USA.
Objective:
Assess the efficiency of seven novel surfactants, relative to the typical Silwet® L-77, for floral dip transformation of Arabidopsis.
Results:
Floral dip transformation of Arabidopsis has been used consistently for 20 years with little change in the protocol. Here we directly compare seven novel surfactants (BREAK-THRU®-OE446, S200, S233, S240, S279, S301 and SP133) to the standard Silwet® L-77 for efficiency of Arabidopsis transformation providing an example of how the surfactants can help other plant transformation protocols. Relative transformation efficiencies ranged from - 44 to + 45% compared to Silwet® L-77. Surfactants S200, S240, and S279 demonstrated the greatest enhancement in transformation.
More Related Videos
08:49Floral-Dip Transformation of Flax Linum usitatissimum to Generate Transgenic Progenies with a High Transformation Rate
Published on: December 19, 2014
06:32Plant Growth and Agrobacterium-mediated Floral-dip Transformation of the Extremophyte Schrenkiella parvula
Published on: January 7, 2019
