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Enzyme-linked immunosorbent assay for determination of rubella IgG antibodies

M Vejtorp

    Acta Pathologica Et Microbiologica Scandinavica. Section B, Microbiology
    |December 1, 1978
    PubMed

    A rubella virus antigen suited for enzyme-linked immunosorbent assay (ELISA) was grown in cultures of BHK/21/13 cells and purified and concentrated by membrane and ultrafiltration. This antigen was used in a semi-automated ELISA for determination of rubella IgG antibodies. The ELISA and the haemagglutination-inhibition (HI) test were compared in a study of 825 human sera. A close correlation was found between the results obtained by the two methods. Antibodies were, however, detected with ELISA in approximately 15% of the sera found negative in the HI test. The presence of antibodies was confirmed by the results of HI tests of the serum fractions of 19 sera separated by rate zonal ultracentrifugation. The EISA procedure employed in the present study was highly sensitive and allowed a precise quantitation of the antibodies by examination of one single serum dilution. ELISA was less time-consuming than the HI test and suited for routine laboratory use.

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    Enzyme-Linked Immunosorbent Assay

    In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
    There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.