Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Introduction to Fibroblasts01:09

Introduction to Fibroblasts

3.2K
Rudolph Virchow discovered spindle-shaped cells called fibroblasts in 1858. Inactive fibroblasts, called fibrocytes, become activated by various stimuli, such as growth factors and inflammatory cytokines. Activated fibroblasts play a crucial role in wound healing, inflammation, formation of new blood vessels, and cancer progression. Uncontrolled activation of fibroblasts results in fibrosis, the excess deposition of fibrous tissue, which can lead to scarring and affect normal organs. This...
3.2K
Overview of Regeneration and Repair01:19

Overview of Regeneration and Repair

4.2K
Regeneration and repair processes are critical in healing damages caused by injury, disease, and aging. In regeneration, the damaged tissue is entirely replaced with new growth that restores the original architecture and function. In contrast, tissue repair usually results in a fixed tissue architecture involving scar formation. Scars generally do not reestablish tissue function and may also exhibit structural abnormalities at the injury site.
Regeneration
All animals have varying degrees of...
4.2K
Renewal of Skin Epidermal Stem Cells01:12

Renewal of Skin Epidermal Stem Cells

2.6K
The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular...
2.6K
Phases of Wound Repair01:28

Phases of Wound Repair

6.2K
Following injury, the integrity of the injured tissues must be reestablished. For example, in skin tissue, wound repair involves coordination among resident skin cells, blood mononuclear cells, extracellular matrix, growth factors, and cytokines to complete the healing cascade.
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...
6.2K
Clinical Applications of Epidermal Stem Cells01:19

Clinical Applications of Epidermal Stem Cells

2.7K
Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own...
2.7K
  1. Home
  3. Fibroblast Inflammatory Priming Determines Regenerative Versus Fibrotic Skin Repair In Reindeer.
  1. Home
  3. Fibroblast Inflammatory Priming Determines Regenerative Versus Fibrotic Skin Repair In Reindeer.

Related Experiment Video

Murine Dermal Fibroblast Isolation by FACS
06:04

Murine Dermal Fibroblast Isolation by FACS

Published on: January 7, 2016

21.4K

Fibroblast inflammatory priming determines regenerative versus fibrotic skin repair in reindeer.

Sarthak Sinha1, Holly D Sparks1, Elodie Labit1

  • 1Faculty of Veterinary Medicine, University of Calgary, Calgary, AB, Canada.

Cell
|December 9, 2022

View abstract on PubMed

Summary
This summary is machine-generated.

Reindeer skin regeneration differs by location: antler velvet regenerates scar-free, while back skin scars. This study reveals fibroblast-immune cell interactions drive these distinct wound healing outcomes.

Keywords:
fetal human fibroblastfibroblastimmune modulationinflammationinflammatory primingmyeloid cell maturationreindeerscarskin regenerationstromal-immune crosstalkwound healing

More Related Videos

Isolation of Papillary and Reticular Fibroblasts from Human Skin by Fluorescence-activated Cell Sorting
09:14

Isolation of Papillary and Reticular Fibroblasts from Human Skin by Fluorescence-activated Cell Sorting

Published on: May 7, 2019

16.0K
Isolation, Culture, and Characterization of Primary Dermal Fibroblasts from Human Keloid Tissue
04:41

Isolation, Culture, and Characterization of Primary Dermal Fibroblasts from Human Keloid Tissue

Published on: July 28, 2023

2.5K

Related Experiment Videos

Murine Dermal Fibroblast Isolation by FACS
06:04

Murine Dermal Fibroblast Isolation by FACS

Published on: January 7, 2016

21.4K
Isolation of Papillary and Reticular Fibroblasts from Human Skin by Fluorescence-activated Cell Sorting
09:14

Isolation of Papillary and Reticular Fibroblasts from Human Skin by Fluorescence-activated Cell Sorting

Published on: May 7, 2019

16.0K
Isolation, Culture, and Characterization of Primary Dermal Fibroblasts from Human Keloid Tissue
04:41

Isolation, Culture, and Characterization of Primary Dermal Fibroblasts from Human Keloid Tissue

Published on: July 28, 2023

2.5K

Area of Science:

  • Regenerative Medicine
  • Comparative Biology
  • Dermatology

Background:

  • Mammalian skin typically heals by forming fibrotic scars, a process that hinders full tissue restoration.
  • Reindeer antler skin (velvet) exhibits scarless regeneration, contrasting with the fibrotic scarring observed in their back skin.

Purpose of the Study:

  • To investigate the cellular and molecular mechanisms underlying the divergent wound healing responses in reindeer antler velvet and back skin.
  • To identify key factors contributing to scarless regeneration versus fibrotic scarring in mammalian skin.

Main Methods:

  • Single-cell multi-omics analysis of fibroblasts from uninjured reindeer velvet and back skin.
  • Assessment of immune cell responses and interactions with fibroblasts following skin injury.
  • Ectopic transplantation experiments to evaluate the plasticity of skin phenotypes.

    • Main Results:

    • Uninjured velvet fibroblasts resemble human fetal fibroblasts, while back skin fibroblasts exhibit pro-fibrotic characteristics.
    • Back skin injury promotes myeloid cell infiltration and maturation, whereas velvet injury leads to an immunosuppressive fibroblast phenotype.
    • Transplanted velvet initially regenerates but transitions to a fibrotic phenotype, mimicking human fetal-to-adult scar transitions.
    • Fibroblast-immune interactions significantly modulate skin regeneration and scarring.

    Conclusions:

    • Reindeer provide a unique model for studying scarless skin regeneration.
    • Site-specific fibroblast phenotypes and their interactions with immune cells dictate wound healing outcomes.
    • Targeting fibroblast-immune crosstalk presents a potential therapeutic strategy for mitigating scar formation and promoting skin regeneration.