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Related Concept Videos

Urethra01:16

Urethra

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The urethra is a hollowed tubular organ through which urine is expelled from the body. This structure extends from the bladder to the external opening, allowing urine to be released.
The anatomy of the urethra differs between males and females. In females, the urethra is short, measuring about 3–4 cm in length, and opens anterior to the vaginal opening. In males, the urethra is longer and passes through the penis, serving dual purposes: expelling urine and ejaculating semen. The male...
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Anatomy of the Genitourinary System II: Bladder and Urethra01:19

Anatomy of the Genitourinary System II: Bladder and Urethra

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The lower urinary system consists of the urinary bladder and urethra, which are essential in storing and expelling urine from the body. Together with the internal and external sphincters, these structures work together to regulate urination effectively.Anatomy of the BladderThe urinary bladder is a muscular, stretchable organ behind the pubic bone and in front of the rectum. In females, the bladder is positioned anterior to the vagina and inferior to the uterus, while in males, it is located...
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Related Experiment Video

Updated: Aug 18, 2025

Surgical Model for Single-Staged Tissue-Engineered Urothelial Tubes in Minipigs
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Engineered human organ-specific urethra as a functional substitute.

Christophe Caneparo1, Stéphane Chabaud1, Julie Fradette1,2

  • 1LOEX/Regenerative Medicine Division, CHU de Québec-Université Laval Research Center, Hôpital Enfant-Jésus, 1401, 18E Rue, Quebec, QC, G1J 1Z4, Canada.

Scientific Reports
|December 9, 2022
PubMed
Summary
This summary is machine-generated.

Engineered urethral tissue using a mix of vesical (VF) and dermal fibroblasts (DF) shows promise for reconstructive surgery. These tissue substitutes offer mechanical strength and functional urothelium, potentially improving patient quality of life.

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Area of Science:

  • Biomaterials Science
  • Urology
  • Tissue Engineering

Background:

  • Urologic pathologies often necessitate surgical reconstruction.
  • A shortage of autologous tissues limits current reconstructive options.
  • Tissue engineering offers a promising alternative for urethral reconstruction.

Purpose of the Study:

  • To develop and clinically characterize engineered urethral tissue substitutes.
  • To optimize the balance between mechanical resistance and urothelial barrier function.
  • To evaluate the potential of fibroblast-seeded constructs for urethral repair.

Main Methods:

  • Fabrication of 3D tissue constructs using vesical fibroblasts (VF) and dermal fibroblasts (DF) in varying ratios (90%:10% and 80%:20%).
  • Assessment of mechanical resistance and functional integrity of engineered tissues.
  • Histological and electron microscopy analysis to evaluate urothelial maturation and tissue characteristics.
  • Quantification of uroplakin and tight junction expression.

Main Results:

  • Tissue substitutes exhibited mechanical resistance comparable to native bladder tissue.
  • Mature urothelium markers (uroplakins, tight junctions) were present in engineered tissues.
  • Maturation correlated positively with increased VF concentration; higher DF content reduced polysaccharide globules.
  • Constructs with at least 10% DF demonstrated adequate mechanical strength and functionality.

Conclusions:

  • Engineered urethral tissue using VF and DF offers a viable alternative for reconstructive surgery.
  • Optimized fibroblast ratios provide mechanical robustness and essential barrier function.
  • These advanced tissue substitutes hold potential for improving long-term patient outcomes in urology.