Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Principles Of Column Chromatography01:13

Principles Of Column Chromatography

7.0K
The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
7.0K
Chromatography: Introduction01:10

Chromatography: Introduction

4.5K
Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
4.5K
High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

2.2K
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
2.2K
Chromatographic Methods: Classification01:12

Chromatographic Methods: Classification

2.4K
Chromatographic techniques are classified in three ways: the classification is based on the physical state of the stationary and mobile phases, how the mobile phase and the stationary phase contact each other, or through the chemical or physical processes that isolate the components of the sample. Typically, the mobile phase is either a liquid or gas, while the stationary phase is either a solid or a liquid layer applied to a solid surface.
Chromatographic techniques are typically named by...
2.4K
Gas Chromatography: Types of Columns and Stationary Phases01:17

Gas Chromatography: Types of Columns and Stationary Phases

834
Gas chromatography (GC) relies on stationary phases to separate and analyze components in a sample. There are two main types of stationary phases: liquid and solid. Liquid stationary phases are non-volatile, thermally stable, and chemically inert liquids coated onto the column. Solid stationary phases are particles of adsorbent material, such as silica gel or molecular sieves.
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
834
Chromatographic Methods: Terminology01:18

Chromatographic Methods: Terminology

2.3K
Chromatography is an analytical technique widely used in fields such as chemistry, biology, environmental science, and pharmaceuticals to separate the components of a mixture and identify substances between them. The process of chromatography is based on the interactions between two distinct phases: the stationary phase and the mobile phase. The stationary phase is fixed in place by a supporting material, while the mobile phase moves over it, carrying the solutes. As the mobile phase travels,...
2.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Maximizing Lipidome Coverage of Mouse Liver Following the IV Administration of Gefitinib by Combining Both UHPLC-MS-Based Untargeted and Targeted Lipidomics.

Journal of proteome research·2026
Same author

Metabolism and Excretion of Synthetic Extended Viperin Pathway Deoxydidehydronucleosides in the Sprague-Dawley Rat.

Journal of proteome research·2026
Same author

Update of descriptors for organosilicon compounds compatible with the 2025-WSU compound descriptor database for use with the solvation parameter model.

Journal of chromatography. A·2026
Same author

Correction: What's in a name? Metabolite identification: challenges and pitfalls in untargeted metabolomics.

Metabolomics : Official journal of the Metabolomic Society·2026
Same author

Addition and update of compounds for the 2025 Wayne state university compound descriptor database for use with the solvation parameter model.

Journal of chromatography. A·2026
Same author

What's in a name? Metabolite identification: challenges and pitfalls in untargeted metabolomics.

Metabolomics : Official journal of the Metabolomic Society·2026
Same journal

Integrated network pharmacology, gut microbiome, and metabolomics analyses reveal the protective mechanism of Guizhi Gegen decoction against influenza.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences·2026
Same journal

Identification of 11α/β-hydroxy-12β-deoxysaxitoxin (12β-deoxyM2) in bivalve molluscs.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences·2026
Same journal

High-resolution LC-MS/MS analysis of brain N-glycans reveals composition-specific changes in Parkinson's disease.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences·2026
Same journal

The discovery of α-glucosidase inhibitors from Folium Mori using virtual screening and ligand fishing strategies.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences·2026
Same journal

Matrix fidelity in microsampling: Plasma-first LC-MS/MS quantification of mycophenolic acid and MPAG.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences·2026
Same journal

Rapid two-step purification of Podoviridae bacteriophages using CIMmultus monolithic chromatography.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences·2026
See all related articles

Related Experiment Video

Updated: Aug 18, 2025

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography
10:14

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

Published on: September 2, 2020

5.1K

Planar chromatography - Current practice and future prospects.

Ian D Wilson1, Colin F Poole2

  • 1Division of Systems Medicine, Department of Metabolism, Digestion and Reproduction, Imperial College, Burlington Danes Building, Du Cane Road, London W12 0NN, UK.

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
|December 10, 2022
PubMed
Summary
This summary is machine-generated.

Thin-layer chromatography (TLC) and high-performance TLC (HPTLC) offer simple, rapid mixture analysis and bioactive component identification. This technique uniquely preserves separations for further component investigation, including quantification and structure determination.

Keywords:
AutomationEffect-directed detectionHPTLCImagingTLCTLC/MS

More Related Videos

Tuning a Parallel Segmented Flow Column and Enabling Multiplexed Detection
08:01

Tuning a Parallel Segmented Flow Column and Enabling Multiplexed Detection

Published on: December 15, 2015

7.5K
Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
06:25

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns

Published on: April 26, 2016

15.2K

Related Experiment Videos

Last Updated: Aug 18, 2025

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography
10:14

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

Published on: September 2, 2020

5.1K
Tuning a Parallel Segmented Flow Column and Enabling Multiplexed Detection
08:01

Tuning a Parallel Segmented Flow Column and Enabling Multiplexed Detection

Published on: December 15, 2015

7.5K
Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
06:25

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns

Published on: April 26, 2016

15.2K

Area of Science:

  • Analytical Chemistry
  • Separation Science

Background:

  • Planar chromatography, encompassing thin-layer chromatography (TLC) and high-performance TLC (HPTLC), remains a vital separation technique.
  • It excels as a straightforward and swift qualitative method for analyzing mixtures and identifying bioactive compounds.

Purpose of the Study:

  • To review current practices and developmental areas in planar chromatography.
  • To outline promising future directions for TLC/HPTLC applications.

Main Methods:

  • Review of existing literature and technological advancements in TLC/HPTLC.
  • Analysis of the unique capabilities of planar chromatography for preserving separated components.

Main Results:

  • TLC/HPTLC provides an unparalleled method for qualitative mixture analysis and bioactive compound discovery.
  • The technique allows for the preservation of separated components, facilitating subsequent detailed analysis (quantification, structure determination).

Conclusions:

  • Planar chromatography continues to be a robust and versatile analytical tool.
  • Future developments in TLC/HPTLC promise expanded applications in various scientific fields.