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A comparison study on polysaccharides extracted from Rosa sterilis S.D.Shi using different methods: Structural and in
Shiguo Chen1,2,3,4,5,6, Luqin Luan1, Yanru Zhang1
1College of Biosystems Engineering and Food Science, National-Local Joint Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang Engineering Laboratory of Food Technology and Equipment, Zhejiang University, Hangzhou 310058, China.
Extraction methods significantly impact Rosa sterilis S.D.Shi polysaccharides (RSP) structure and probiotic effects. Different RSPs modulate gut microbiota by promoting beneficial bacteria and inhibiting pathogens, offering insights for functional food development.
Area of Science:
- Food Science
- Microbiology
- Biochemistry
Background:
- Polysaccharides from Rosa sterilis S.D.Shi (RSP) are under investigation for their potential health benefits.
- Understanding the structure-function relationship of RSPs is crucial for developing functional foods.
Purpose of the Study:
- To characterize the structural and in vitro fermentation properties of RSPs extracted using different methods (hot water, acid, alkali, enzyme).
- To evaluate the impact of extraction methods on the probiotic effects of RSPs.
Main Methods:
- Extraction of RSPs using hot water, acid, alkali, and enzyme methods.
- Structural characterization including monosaccharide composition and molecular weight determination.
- In vitro fermentation assays to assess effects on gut microbiota composition and short-chain fatty acid (SCFA) production.
Main Results:
- Extraction methods significantly altered RSP structure, including monosaccharide profiles (Gal, Glc, GalA, Ara) and molecular weights (ranging from 57.55 kDa to 922.20 kDa).
- Enzyme-extracted RSP (EM-RSP) was rich in RG-I with larger side chains.
- All RSPs promoted beneficial bacteria (Bifidobacterium, Bacteroides, Faecalibacterium, Paraclostrium) and SCFAs production, while inhibiting pathogenic bacteria (Escherichia-shigella).
- EM-RSP showed unique metabolic pathways.
Conclusions:
- Extraction method is a key determinant of RSP structure and subsequent probiotic functionality.
- RSPs possess the potential to modulate gut microbiota composition and function.
- Findings provide a theoretical basis for developing functional products from Rosa sterilis S.D.Shi.
