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To Explore the Inhibitory Mechanism of Quercetin in Thyroid Papillary Carcinoma through Network Pharmacology and

Ying Sun1,2, Wenjun Xie1,3, Ning Kang1

  • 1Department of Thyroid and Neck Tumor, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer, Tianjin 300060, China.

Disease Markers
|December 13, 2022
PubMed

Insights

Quercetin shows potential as an anticancer agent for papillary thyroid carcinoma (PTC). This study identified key molecular targets and signaling pathways, like TNF and PI3K-AKT, involved in quercetin

Area of Science:

  • * Pharmacology
  • * Molecular Biology
  • * Bioinformatics

Background:

  • * Quercetin, a flavonoid, exhibits anti-inflammatory and anticancer properties.
  • * Its therapeutic potential for papillary thyroid carcinoma (PTC) is promising but requires mechanistic elucidation.
  • * Downstream signaling pathways mediating quercetin's anticancer effects in PTC are not fully understood.

Purpose of the Study:

  • * To investigate the molecular targets and signaling pathways of quercetin in papillary thyroid carcinoma (PTC).
  • * To evaluate the anticancer efficacy of quercetin in PTC cell lines using network pharmacology.
  • * To provide a mechanistic basis for quercetin's application in PTC treatment.

Main Methods:

  • * Bioinformatic screening using PharmMapper and Gene Expression Omnibus (GEO) database.
  • * Protein-protein interaction (PPI) network construction and analysis.
  • * Molecular docking, western blot, CCK-8, transwell, flow cytometry, and colony formation assays.

Main Results:

  • * Four core targets (MMP9, JUN, SPP1, HMOX1) were identified through PPI network analysis.
  • * These targets are enriched in TNF, PI3K-AKT, and NF-κB signaling pathways, crucial for tumor development.
  • * Molecular docking confirmed strong binding affinity between quercetin and the identified protein targets.
  • * Quercetin demonstrated significant antitumor efficacy in PTC cell lines.

Conclusions:

  • * Network pharmacology effectively identified quercetin's molecular targets and pathways in PTC.
  • * Quercetin regulates multiple proteins and signaling pathways, inhibiting PTC progression.
  • * These findings support quercetin as a potential therapeutic agent for papillary thyroid carcinoma.

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