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Updated: Aug 17, 2025

Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass
Published on: August 10, 2017
Analysis of Golgi Morphology Using Immunofluorescence and CellProfiler Software
Isabel Mejia1, Yu-Chuan Chen1, Begoña Díaz2,3
1Department of Internal Medicine, Division of Medical Hematology and Oncology, The Lundquist Institute for Biomedical Innovation at Harbor-UCLA Medical Center, Torrance, CA, USA.
Abstract:
The architecture of the Golgi apparatus in mammalian cells changes dynamically in response to internal and external cues and may be permanently altered in disease states. Here, we present a method to quantify changes in Golgi morphology using immunofluorescence and confocal microscopy followed by CellProfiler software analysis. This method will assist researchers in evaluating alterations in the Golgi complex morphology of cultured cells under a variety of different experimental conditions.
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