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Updated: Aug 17, 2025
08:49
Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens
Published on: June 6, 2020
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Engineered cell entry links receptor biology with single-cell genomics
Bingfei Yu1, Quanming Shi2, Julia A Belk3
1Center for Personal Dynamic Regulomes, Stanford University, Stanford, CA 94305, USA.
Cell
|December 14, 2022
Summary
Engineered receptor-ligand interaction (ENTER) technology decodes cellular communication and enables targeted payload delivery. This method precisely maps antigen specificities and cell fates, advancing immunology research.
Area of Science:
- Immunology
- Molecular Biology
- Biotechnology
Background:
- Cellular communication relies on receptor-ligand interactions.
- Understanding these interactions is crucial for immunology and disease research.
Purpose of the Study:
- To develop a novel technology, ENTER (engineered receptor-ligand interaction), for studying receptor-ligand interactions.
- To enable targeted payload delivery and precise recording of cellular responses.
Main Methods:
- Lentiviral-mediated display of engineered receptor-ligand interactions.
- Optimization for T cell receptor (TCR)-MHC peptide and antibody-antigen pairs.
- Single-cell RNA sequencing (ENTER-seq) for multiplexed analysis.
Main Results:
- ENTER successfully decodes various receptor-ligand pairs, including TCR-MHC and antibody-antigen interactions.
- ENTER-seq enables detailed analysis of T cell antigen specificities, clonality, and cell states.
- Analysis of patient samples revealed viral epitopes driving T cell differentiation and diversity.
Conclusions:
- ENTER technology provides a versatile platform for discovering receptor specificities and their functional consequences.
- This system facilitates antigen-specific delivery of genetic payloads to modulate cellular behavior.
- ENTER-seq offers a powerful tool for dissecting immune responses at the single-cell level.
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