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Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines
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Published on: June 2, 2018

Mapping enhancer-gene regulatory interactions from single-cell data

Maya U Sheth1,2,3,4, Wei-Lin Qiu1,5, X Rosa Ma2,3

  • 1The Novo Nordisk Foundation Center for Genomic Mechanisms of Disease, Broad Institute of MIT and Harvard, Cambridge, MA, USA.

Nature Genetics
|August 3, 2026
PubMed
Summary

No abstract available in PubMed .

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Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...