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Characterization of Immune Cell-derived Extracellular Vesicles and Studying Functional Impact on Cell Environment
Published on: June 2, 2020
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Human amniotic fluid derived extracellular vesicles attenuate T cell immune response
Tania Del Rivero1, Julian Milberg1, Cassie Bennett1
1Organicell Regenerative Medicine, Miami, FL, United States.
Frontiers in Immunology
|December 15, 2022
Summary
Human amniotic fluid extracellular vesicles (AF-EVs) and acellular amniotic fluid (acAF) suppress T cell proliferation and activation. AF-EVs show potent immunomodulatory effects, suggesting their potential as an immunotherapy for T cell-mediated conditions.
Area of Science:
- Immunology
- Cell Biology
- Biotechnology
Background:
- Extracellular vesicles from human amniotic fluid (AF-EVs) modulate inflammation.
- The impact of AF-EVs and acellular amniotic fluid (acAF) on T cell immunity remains unexplored.
Purpose of the Study:
- To investigate the immunomodulatory effects of acAF and AF-EVs on T cell responses.
- To determine if AF-EVs can be utilized as an immunotherapeutic agent.
Main Methods:
- Peripheral Blood Mononuclear Cells (PBMCs) were stimulated with Phytohemagglutinin (PHA).
- Cells were treated with vehicle, acAF, or concentrated AF-EVs.
- T cell proliferation, activation, and cytokine release were measured.
Main Results:
- Both acAF and AF-EVs suppressed PHA-induced T cell proliferation and activation.
- AF-EVs demonstrated a more significant suppressive effect than acAF.
- Pro-inflammatory cytokine release from PBMCs was reduced by both treatments.
- AF-EVs were internalized by CD4+ and CD8+ effector T cells.
Conclusions:
- AF-EVs possess robust immunomodulatory properties on T cells.
- AF-EVs hold promise as a potential immunotherapeutic tool for T cell-related disorders.
