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Insane in the vembrane: filtering and transforming VCF/BCF files
Till Hartmann1, Christopher Schröder2, Elias Kuthe3
1Algorithms for Reproducible Bioinformatics, University Hospital Essen, University of Duisburg-Essen, Essen 45147, Germany.
Summary:
We present vembrane as a command line variant call format (VCF)/binary call format (BCF) filtering tool that consolidates and extends the filtering functionality of previous software to meet any imaginable filtering use case. Vembrane exposes the VCF/BCF file type specification and its inofficial extensions by the annotation tools VEP and SnpEff as Python data structures. vembrane filter enables filtration by Python expressions, requiring only basic knowledge of the Python programming language. vembrane table allows users to generate tables from subsets of annotations or functions thereof. Finally, it is fast, by using pysam and relying on lazy evaluation.
Availability And Implementation:
Source code and installation instructions are available at github.com/vembrane/vembrane (doi: 10.5281/zenodo.7003981).
Supplementary Information:
Supplementary data are available at Bioinformatics online.
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