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Updated: Aug 17, 2025

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform
Published on: January 30, 2017
An updated protocol for in vitro bovine embryo production
Savannah L Speckhart1, Lydia K Wooldridge1, Alan D Ealy1
1School of Animal Sciences, Blacksburg, VA 24060, USA.
Abstract:
Cattle embryos represent a useful model for understanding parts of human embryogenesis due to various biological similarities. We describe a protocol to mature and fertilize bovine oocytes followed by culture of resulting presumptive zygotes up until the blastocyst stage. Our protocol features a unique procedure for washing and moving oocytes and zygotes between their respective dishes using a cell strainer. A thorough troubleshooting section will help users optimize embryo development with cleavage and blastocyst rates exceeding 70% and 20%, respectively. For complete details on the use and execution of this protocol, please refer to Wooldridge and Ealy (2019).1.

