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Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform
Published on: January 30, 2017
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An updated protocol for in vitro bovine embryo production
Savannah L Speckhart1, Lydia K Wooldridge1, Alan D Ealy1
1School of Animal Sciences, Blacksburg, VA 24060, USA.
STAR Protocols
|December 15, 2022
Summary
This study details a cattle embryo development protocol, offering a model for human embryogenesis research. The optimized method achieves high cleavage and blastocyst rates, aiding reproductive science advancements.
Area of Science:
- Reproductive Biology
- Developmental Biology
- Comparative Embryology
Background:
- Cattle embryos serve as valuable models for studying human embryogenesis due to conserved biological processes.
- Understanding early embryonic development in cattle can provide insights into fundamental mechanisms of mammalian development.
Purpose of the Study:
- To describe a detailed protocol for in vitro maturation and fertilization of bovine oocytes.
- To outline the subsequent culture of presumptive zygotes to the blastocyst stage.
- To provide a reliable method for optimizing cattle embryo development.
Main Methods:
- Maturation and in vitro fertilization of bovine oocytes.
- Culture of presumptive zygotes to the blastocyst stage.
- Utilized a unique cell strainer method for oocyte and zygote transfer between culture dishes.
Main Results:
- Achieved cleavage rates exceeding 70%.
- Achieved blastocyst development rates exceeding 20%.
- Protocol includes troubleshooting guidance for optimization.
Conclusions:
- The described protocol provides an effective method for cattle embryo development.
- This protocol can be utilized for research in comparative embryology and reproductive technologies.
- Optimized embryo development rates support the utility of this model system.
