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Atomic emission spectroscopy (AES) is an analytical technique used to determine the elemental composition of a sample by analyzing the light emitted from excited atoms. In AES, atoms in a sample are excited to higher energy levels by thermal energy from high-temperature sources, such as plasma, arcs, or sparks. When these excited atoms return to lower energy states, they emit light at specific wavelengths characteristic of each element. The resulting atomic emission spectrum, which consists of...
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In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis
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Quantitative Element-Sensitive Analysis of Individual Nanoobjects.

André Wählisch1, Rainer Unterumsberger1, Philipp Hönicke1

  • 1Physikalisch-Technische Bundesanstalt (PTB), Abbestr. 2-12, 10587, Berlin, Germany.

Small (Weinheim an Der Bergstrasse, Germany)
|December 15, 2022
PubMed
Summary
This summary is machine-generated.

Traceable quantification of individual nanoobjects is possible using X-ray fluorescence microscopy with well-calibrated instruments. This method avoids the need for reference nanomaterials, enabling accurate analysis of advanced materials.

Keywords:
nanobeam X-ray fluorescence (XRF)nanometrologynanostructure characterizationquantitative analysis

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Area of Science:

  • Materials Science
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Quantitative material analysis is vital for new technologies, particularly at the nanoscale.
  • X-ray fluorescence microscopy (XFM) is an element-sensitive, non-destructive technique for nanomaterials.
  • Current XFM quantification is often limited to qualitative or semi-quantitative analysis at the nanoscale.

Purpose of the Study:

  • To demonstrate traceable quantification of individual nanoobjects using XFM.
  • To overcome limitations of reference nanomaterial dependency in nanoscale analysis.
  • To establish a method for accurate atom counting in nanoobjects.

Main Methods:

  • Utilized a well-calibrated X-ray fluorescence microscope.
  • Employed soft X-ray radiation for analysis.
  • Reconstructed complementary dimensional parameters of nanoobjects.

Main Results:

  • Achieved traceable quantification of the total number of atoms in a germanium nanoobject.
  • Demonstrated the feasibility of quantitative analysis without reference nanomaterials.
  • Successfully reconstructed dimensional parameters of the analyzed nanoobjects.

Conclusions:

  • Traceable quantification of individual nanoobjects is achievable with conventional, well-calibrated XFM instrumentation.
  • This approach provides a reliable alternative to reference materials for nanoscale analysis.
  • Enables accurate assessment of advanced nanotechnological materials.