Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.5K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.5K
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

8.3K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
8.3K
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

5.0K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
5.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Monodisperse Octahedral PbSe Nanocrystals Encased in Eight (111) Facets and Their Orthorhombic Superlattice.

Journal of the American Chemical Society·2026
Same author

Global research trends in programmed cell death in rheumatoid arthritis from 2001 to 2025: a bibliometric analysis.

Frontiers in immunology·2026
Same author

Astrocytic FDX1 Contributes to Copper Dyshomeostasis-associated Synaptic Dysfunction in Depression and Is Modulated by Exercise.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Freeform holographic curved-waveguide retinal projection near-eye display.

Optics express·2026
Same author

Design and optimization of compact LCoS projection optics using a freeform PBS prism.

Optics express·2026
Same author

Association between pre-pregnancy body mass index and neonatal outcomes in women undergoing assisted reproductive technology: a retrospective study.

BMC pregnancy and childbirth·2026
Same journal

Long-term stabilization of intensity-difference squeezing from four-wave mixing in rubidium vapor.

Optics express·2026
Same journal

Robust 3D topography measurement of large-range high-aspect-ratio structures based on dual-domain statistical filtering in SD-OCT.

Optics express·2026
Same journal

Broadband transmissive terahertz metasurface for simultaneous quad-mode OAM multiplexing.

Optics express·2026
Same journal

Leveraging two-dimensional materials for high-sensitivity optical sensors: quasi-bound states in the continuum within hybrid metasurfaces.

Optics express·2026
Same journal

Resolution investigation for dual-spherical-wave optical scanning holographic microscopy: methods and performance.

Optics express·2026
Same journal

Robustness of parallel subnetwork-filtered diffractive deep neural networks.

Optics express·2026
See all related articles

Related Experiment Video

Updated: Aug 17, 2025

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
08:39

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Published on: January 28, 2019

9.9K

Design method of imaging optical systems using confocal flat phase elements.

Tong Yang, Lina Gao, Dewen Cheng

    Optics Express
    |December 16, 2022
    PubMed
    Summary
    This summary is machine-generated.

    A new design method for flat phase imaging systems, including diffractive optical elements and metasurfaces, offers efficient, generalized solutions for stigmatic imaging, even in nonsymmetric configurations. This approach simplifies optical system design and accelerates the development of advanced imaging technologies.

    More Related Videos

    Video-rate Scanning Confocal Microscopy and Microendoscopy
    14:10

    Video-rate Scanning Confocal Microscopy and Microendoscopy

    Published on: October 20, 2011

    28.0K
    Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
    10:28

    Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

    Published on: July 5, 2016

    10.4K

    Related Experiment Videos

    Last Updated: Aug 17, 2025

    Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
    08:39

    Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

    Published on: January 28, 2019

    9.9K
    Video-rate Scanning Confocal Microscopy and Microendoscopy
    14:10

    Video-rate Scanning Confocal Microscopy and Microendoscopy

    Published on: October 20, 2011

    28.0K
    Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
    10:28

    Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

    Published on: July 5, 2016

    10.4K

    Area of Science:

    • Optical Engineering
    • Metasurface Optics
    • Diffractive Optics

    Background:

    • Flat phase elements like diffractive optical elements (DOEs), holographic optical elements (HOEs), and metasurfaces are crucial for modern imaging systems.
    • Existing design methods often lack generality and efficiency, particularly for nonsymmetric optical configurations.

    Purpose of the Study:

    • To propose a generalized and efficient design method for imaging systems utilizing flat phase elements.
    • To enable fast calculation of phase functions for stigmatic imaging in both symmetric and nonsymmetric systems.

    Main Methods:

    • Derivation of a generalized phase function based on confocal properties for point-to-point stigmatic imaging.
    • Calculation of phase functions using specified focal length or magnification and element locations.
    • Demonstration of design examples to validate the method's feasibility.

    Main Results:

    • Achieved fast and accurate calculation of phase functions for stigmatic imaging.
    • Successfully demonstrated the method's effectiveness through various design examples.
    • The designed systems serve as excellent starting points for further optimization with additional phase terms.

    Conclusions:

    • The proposed method significantly enhances design efficiency for flat phase imaging systems.
    • It reduces reliance on pre-existing systems and specialized expertise.
    • The method is readily integrable into standard optical design software.