Deep learning based protocol to construct an immune-related gene network of host-pathogen interactions in plants
1Functional Genomics and Complex System Lab, Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology (CSIR-IHBT), Palampur, Himachal Pradesh, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India.
Abstract:
Investigating network behavior from host-pathogen interactions is challenging. Here, we present the deep-learning-based protocol to construct an immune-related gene network and list the genes involved in the defense response of host to specific biotic stress. The protocol includes the steps to pre-process the interaction pairs and expression profile of plants treated with pathogen/control, feed as input for DLNet algorithm to rank genes based on their contribution to data classification. The top-ranked genes are subjected to module and enrichment analysis. For complete details on the use and execution of this protocol, please refer to Kumar et al. (2022).1.
Related Concept Videos
02:26Defenses Against Pathogens and Herbivores
01:29Immune Response Against Viral Pathogens
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...