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Updated: Aug 17, 2025
Registered Bioimaging of Nanomaterials for Diagnostic and Therapeutic Monitoring
Published on: December 9, 2010
Detection of SARS-CoV-2 Nucleocapsid and Microvascular Disease in the Brain: A Case Report
Catherine DeMarino1, Myoung-Hwa Lee1, Maria Cowen1
1From the Section for Infections of the Nervous System (C.D., M.-H.L., M.C., A.N.), NeuroTherapeutics Development Unit (J.P.S.), Translational Neuroscience Center, Neurophysiology of Epilepsy Unit (S.I.), and Surgical Neurology Branch (A.H.S., K.A.Z.), National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD.
Background And Objectives:
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection can cause a wide range of neurologic complications; however, its neuropenetrance during the acute phase of the illness is unknown.
Methods:
Extracellular vesicles were isolated from brain biopsy tissue from a patient undergoing epilepsy surgery using ultracentrifugation and analyzed by Western blot and qPCR for the presence of virus protein and RNA, respectively. Biopsy tissue was assessed by immunohistochemistry for the presence of microvascular damage and compared with 3 other non-COVID surgical epilepsy brain tissues.
Results:
We demonstrate the presence of viral nucleocapsid protein in extracellular vesicles and microvascular disease in the brain of a patient undergoing epilepsy surgery shortly after SARS-CoV-2 infection. Endothelial cell activation was indicated by increased levels of platelet endothelial cell adhesion molecule-1 and was associated with fibrinogen leakage and immune cell infiltration in the biopsy tissue as compared with control non-COVID surgical epilepsy brain tissues.
Discussion:
Despite the lack of evidence of viral replication within the brain, the presence of the nucleocapsid protein was associated with disease-specific endothelial cell activation, fibrinogen leakage, and immune cell infiltration.
