Colorimetric detection of Cu²⁺ based on the inhibition strategy for etching reaction of AgNCs

In this study, an efficient and simple colorimetric method for sensing of Cu²⁺ was established using inhibition effect of Cu²⁺ to the etching reaction of silver nanocubes (AgNCs) induced by H₂O₂. The etching reaction of AgNCs changes its morphology and absorbance with the visual appearance from yellow to colorless. On the contrary, the presence of Cu²⁺ can significantly inhibit the etching progress. Thus Cu²⁺ induces the obvious absorbance enhancement compared with AgNCs/H₂O₂ system. This design realizes colorimetric detection of Cu²⁺ based on the inhibition effect of etching reaction using AgNCs nanoprobe. The colorimetric response of AgNCs nanoprobe in ΔAbs_417.5 shows the linearity with the increasing concentrations of Cu²⁺ from 0.01 to 40 μM with good selectivity. The concentration limit of Cu²⁺ efficaciously discriminated by the naked eye is as low as 0.01 μM. Furthermore, the Euclidean distance (ED) of the difference map in RGB change before and after response with Cu²⁺ is applied for further visualization recognition of Cu²⁺. All the above results indicate the outstanding practicability and accuracy of the proposed assay for Cu²⁺ sensing.