Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Age-related decrease of growth hormone and prolactin gene expression in the mouse pituitary.

M D Crew1, S R Spindler, R L Walford

  • 1Department of Biochemistry, University of California, Riverside 92521.

Endocrinology
|October 1, 1987
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genetic diversity of Lactobacillus delbrueckii isolated from raw milk in Hokkaido, Japan.

Journal of dairy science·2021
Same author

Diet-induced alteration of fatty acid synthase in prostate cancer progression.

Oncogenesis·2016
Same author

Coping strategies and risk of cardiovascular disease incidence and mortality: the Japan Public Health Center-based prospective Study.

European heart journal·2016
Same author

Utilization of native oxygen in Eu(RE)-doped GaN for enabling device compatibility in optoelectronic applications.

Scientific reports·2016
Same author

Detection of methicillin-resistant Staphylococcus pseudintermedius ST169 and novel ST354 SCCmec II-III isolates related to the worldwide ST71 clone.

Epidemiology and infection·2015
Same author

Cognitive and affective impairments of a novel SCA/MND crossroad mutation Asidan.

European journal of neurology·2012
Same journal

Zingerone ameliorates ovarian impairment by regulating steroidogenesis and apoptosis in letrozole-treated mouse.

Endocrinology·2026
Same journal

Loss of the calorie restriction response protein DEPP1 worsens diet-induced obesity.

Endocrinology·2026
Same journal

Long noncoding RNAs mediate endocrine signaling and resistance in prostate cancer.

Endocrinology·2026
Same journal

Repeated Chemogenetic Activation of C1 Catecholamine Neurons Reduces Subsequent Glucoprivic Responses and Mimics HAAF.

Endocrinology·2026
Same journal

Postnatal exposure to maternal hypothyroidism leads to developmental delay and metabolic dysregulations in male mice.

Endocrinology·2026
Same journal

Discovery proteomics identification of factors contributing to gonadotropin β expression.

Endocrinology·2026
See all related articles

Aging significantly reduces pituitary growth hormone (GH) and prolactin (PRL) mRNA levels in mice. This decline is specific, as other messenger RNA (mRNA) levels remain unchanged, suggesting age-related factors beyond T3 influence gene expression.

Area of Science:

  • Endocrinology
  • Molecular Biology
  • Aging Research

Background:

  • Pituitary hormones like growth hormone (GH) and prolactin (PRL) play crucial roles in physiological functions.
  • Age-related changes in gene expression can impact hormone production and secretion.
  • Understanding molecular mechanisms underlying aging is vital for healthspan research.

Purpose of the Study:

  • To investigate the impact of aging on pituitary growth hormone (GH) and prolactin (PRL) messenger RNA (mRNA) levels in male mice.
  • To determine if age-related changes in GH and PRL mRNA are specific or widespread across cellular mRNAs.
  • To explore potential correlations between age-related hormone mRNA decline and serum T3 levels.

Main Methods:

  • Utilized dot-blot hybridization to quantify GH, PRL, and alpha-tubulin mRNA levels in pituitaries of 3-, 12-, and 27-month-old male C57BL/6J mice.

Related Experiment Videos

  • Assessed total poly(A+) RNA (mRNA) levels using hybridization with radioactively labeled oligo-(dT).
  • Measured serum T3 levels to evaluate its potential role in age-related gene expression changes.

    • Main Results:

    • A dramatic age-dependent decrease in pituitary GH and PRL mRNA levels was observed.
    • GH mRNA levels dropped by 35% between 3 and 12 months, and 75% by 27 months.
    • PRL mRNA levels decreased by 65% between 12 and 27 months, with no significant change before 12 months.
    • Total mRNA and alpha-tubulin mRNA levels showed no significant age-related alterations.
    • No significant age-related changes were detected in serum T3 levels.

    Conclusions:

    • Aging specifically reduces GH and PRL mRNA levels in the pituitary gland of male mice.
    • The observed decline in GH and PRL mRNA is not indicative of a general age-related decrease in cellular mRNA.
    • The results suggest that factors other than T3 are involved in the age-related decline of GH and PRL gene expression.