Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structure-Function Correlation With Optical Coherence Tomography-Derived Macular Thickness and Macular Microperimetry in Children, Adolescents, and Young Adults With Sickle Cell Disease.

Journal of vitreoretinal diseases·2026
Same author

Rates, Timing, and Predictors of Retreatment Across Risk-Cohorts in Retinopathy of Prematurity: Intravitreal Bevacizumab Injection vs Laser.

American journal of ophthalmology·2026
Same author

Rapid Macular Thinning as a Biomarker for Geographical Atrophy.

Clinical ophthalmology (Auckland, N.Z.)·2026
Same author

Perivascular Macrophages Are Associated With Permissive Sites of Immune Cell Extravasation in Retinal Venules.

Investigative ophthalmology & visual science·2026
Same author

GLP-1 Receptor Agonist Treatment and Health Outcomes in Methadone-Treated Patients with Opioid Use Disorder and Diabetes.

Journal of general internal medicine·2026
Same author

Real-world outcomes of retinopathy of prematurity treated with primary laser versus primary anti-vascular endothelial growth factor and deferred laser.

Journal of AAPOS : the official publication of the American Association for Pediatric Ophthalmology and Strabismus·2026

Related Experiment Video

Updated: Aug 16, 2025

An Alternative and Validated Injection Method for Accessing the Subretinal Space via a Transcleral Posterior Approach
07:13

An Alternative and Validated Injection Method for Accessing the Subretinal Space via a Transcleral Posterior Approach

Published on: December 7, 2016

13.4K

Deformation of Aflibercept and Ranibizumab Syringes Causes Variation in Intravitreal Injection Volume and Risks

Joseph J Raevis1,2, Colin A Lemire1, David J Ramsey3,4

  • 1Department of Ophthalmology, Beth Israel Deaconess Medical Center, Boston, Massachusetts.

Ophthalmology Science
|December 19, 2022
PubMed
Summary

Intravitreal injection volume can increase due to syringe stopper deformation when excessive force is applied. Avoid forceful injections and releasing plunger pressure in the eye to prevent over-dosing and vitreous incarceration.

Keywords:
CI, confidence intervalIntravitreal injectionPFS, prefilled syringeSyringeTB, tuberculinVariabilityVolumeaflibercept

More Related Videos

Subretinal Transplantation of Human Embryonic Stem Cell Derived-retinal Pigment Epithelial Cells into a Large-eyed Model of Geographic Atrophy
11:03

Subretinal Transplantation of Human Embryonic Stem Cell Derived-retinal Pigment Epithelial Cells into a Large-eyed Model of Geographic Atrophy

Published on: January 22, 2018

10.1K
Retinal Pigment Epithelium Transplantation in a Non-human Primate Model for Degenerative Retinal Diseases
11:20

Retinal Pigment Epithelium Transplantation in a Non-human Primate Model for Degenerative Retinal Diseases

Published on: June 14, 2021

3.9K

Related Experiment Videos

Last Updated: Aug 16, 2025

An Alternative and Validated Injection Method for Accessing the Subretinal Space via a Transcleral Posterior Approach
07:13

An Alternative and Validated Injection Method for Accessing the Subretinal Space via a Transcleral Posterior Approach

Published on: December 7, 2016

13.4K
Subretinal Transplantation of Human Embryonic Stem Cell Derived-retinal Pigment Epithelial Cells into a Large-eyed Model of Geographic Atrophy
11:03

Subretinal Transplantation of Human Embryonic Stem Cell Derived-retinal Pigment Epithelial Cells into a Large-eyed Model of Geographic Atrophy

Published on: January 22, 2018

10.1K
Retinal Pigment Epithelium Transplantation in a Non-human Primate Model for Degenerative Retinal Diseases
11:20

Retinal Pigment Epithelium Transplantation in a Non-human Primate Model for Degenerative Retinal Diseases

Published on: June 14, 2021

3.9K

Area of Science:

  • Ophthalmology
  • Biomedical Engineering
  • Drug Delivery Systems

Background:

  • Intravitreal injection volume is susceptible to variations influenced by plunger alignment and injection speed.
  • Syringe stopper deformation is a potential factor contributing to inaccurate dosing during intravitreal injections.

Purpose of the Study:

  • To investigate the role of syringe stopper deformation in enabling excess intravitreal injection volumes.
  • To assess the risk of vitreous humor incarceration due to released force within the eye.

Main Methods:

  • Experimental study involving aflibercept and ranibizumab prefilled syringes (PFSs) and tuberculin (TB) syringes.
  • Subjecting syringes to increasing injection force to measure excess volume expulsion beyond the intended 50-μl dose.
  • Assessing volume changes upon release of applied force.

Main Results:

  • Syringe stoppers deform into the dead space under additional force, leading to increased medication doses.
  • At 3.92 N additional force, aflibercept PFSs, ranibizumab PFSs, and TB syringes dispensed 17.2%, 11.4%, and 0.8% more volume than intended, respectively.
  • A proportional volume was drawn back into the needle upon release of the applied force.

Conclusions:

  • Intravitreal injection volume is significantly affected by syringe stopper deformation caused by applied force.
  • Forceful intravitreal injections should be avoided to prevent medication over-dosing.
  • Releasing plunger pressure while the needle is in the vitreous cavity can cause vitreous incarceration, potentially leading to retinal tears or detachment.