Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements

Area of Science:

• Biophysics
• Cell Biology
• Microfluidics

Background:

• Cellular mechanical properties influence diverse biological processes and diseases.
• Conventional methods (AFM, MA) offer rich data but low throughput.
• High-throughput methods (RT-DC) provide limited mechanical insights.

Purpose of the Study:

• To introduce mechano-node-pore sensing (mechano-NPS) as a microfluidic platform.
• To bridge the gap between low-throughput, high-information methods and high-throughput, low-information methods.
• To enable multi-parameter viscoelastic measurements of cells at moderate throughput.

Main Methods:

• Utilizes a direct current (DC) measurement in a microfluidic channel.
• Monitors cell size and velocity during transit through a narrow constriction.
• Quantifies transverse deformation, resistance, and recovery from deformation.

Main Results:

• Provides multi-parameter viscoelastic cell properties for a comprehensive mechanical state assessment.
• Offers a flexible, label-free, and electronics-based microfluidic sensing approach.
• Demonstrates simple implementation, minimal sample preparation, and adaptability.

Conclusions:

• Mechano-NPS offers a versatile and accessible platform for detailed cellular mechanical analysis.
• The platform provides unique mechanical information for diverse cell types.
• Has potential applications in basic science research and clinical diagnostics.