Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genomics02:02

Genomics

36.8K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
36.8K
Applications of Molecular Taxonomy01:20

Applications of Molecular Taxonomy

59
Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
59
Genome-wide Association Studies-GWAS01:11

Genome-wide Association Studies-GWAS

13.9K
Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more frequently observed in individuals with a particular disease than those without the disease. In that case, those SNPs are said to be associated with the disease. Chi-square analysis is performed to check the probability of the allele likely to be associated with the disease.
GWAS does not require the identification of the target gene involved in...
13.9K
Multi-species Conserved Sequences02:51

Multi-species Conserved Sequences

4.0K
Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale  studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved...
4.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identification of Viral Variants from Functional Genomics Data.

F1000Research·2026
Same author

Phosphorylation of SF3B1 by CDK11 orchestrates spliceosome activation via SNIP1-dependent RES complex recruitment.

Nature communications·2026
Same author

Retention of embryonic positional identity signatures in the adult sheep tail: evidence from HOXB13 spatial RNA expression gradients.

Scientific reports·2026
Same author

Exonuclease ISG20 inhibits human cytomegalovirus replication by inducing an innate immune defense signature.

PLoS pathogens·2026
Same author

CDK7-CDK11 axis in spliceosome regulation and pre-mRNA splicing.

Nucleic acids research·2025
Same author

Whole blood RNA profiling in cats dissects the host immunological response during recovery from feline infectious peritonitis.

PloS one·2025
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Aug 16, 2025

Author Spotlight: Integrated Multi-Omics Analysis for Unveiling Multicellular Immune Signatures in Clinical Heart Attack Cohorts
08:51

Author Spotlight: Integrated Multi-Omics Analysis for Unveiling Multicellular Immune Signatures in Clinical Heart Attack Cohorts

Published on: September 20, 2024

1.4K

Computational Integration of HSV-1 Multi-omics Data.

Caroline C Friedel1

  • 1Institute of Informatics, Ludwig-Maximilians-Universität München, Munich, Germany. caroline.friedel@bio.ifi.lmu.de.

Methods in Molecular Biology (Clifton, N.J.)
|December 19, 2022
PubMed
Summary
This summary is machine-generated.

Herpes simplex virus 1 (HSV-1) infection disrupts host gene transcription termination, causing widespread read-through transcription. This functional genomics study reveals novel viral infection mechanisms impacting host chromatin architecture.

Keywords:
4sU-seqATAC-seqBioinformaticsChromatin accessibilityDisruption of transcription terminationHerpes simplex virus 1RNA-seqRead-through transcriptionRibosome profilingTranscriptionTranslationVirus-host interactions

More Related Videos

Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining
13:22

Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining

Published on: January 23, 2014

18.4K
High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions
14:58

High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions

Published on: March 5, 2022

4.4K

Related Experiment Videos

Last Updated: Aug 16, 2025

Author Spotlight: Integrated Multi-Omics Analysis for Unveiling Multicellular Immune Signatures in Clinical Heart Attack Cohorts
08:51

Author Spotlight: Integrated Multi-Omics Analysis for Unveiling Multicellular Immune Signatures in Clinical Heart Attack Cohorts

Published on: September 20, 2024

1.4K
Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining
13:22

Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining

Published on: January 23, 2014

18.4K
High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions
14:58

High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions

Published on: March 5, 2022

4.4K

Area of Science:

  • Molecular Biology
  • Virology
  • Genomics

Background:

  • Next-generation sequencing offers advanced functional genomics for studying host responses to viral infections.
  • Understanding host-pathogen interactions at transcriptional, translational, and chromatin levels is crucial.

Purpose of the Study:

  • To provide an overview of computational integration of multi-omics data in lytic herpes simplex virus 1 (HSV-1) infection.
  • To summarize methods for analyzing de novo transcription, active translation, transcript localization, and chromatin accessibility.

Main Methods:

  • Utilized 4sU-seq for de novo transcription analysis.
  • Employed ribosome profiling to investigate active translation.
  • Applied RNA-seq on subcellular fractions for transcript localization.
  • Performed ATAC-seq for genome-wide chromatin accessibility profiling.

Main Results:

  • HSV-1 infection causes unprecedented disruption of host transcription termination.
  • Widespread read-through transcription beyond poly(A) sites was observed for most host genes.
  • Increased chromatin accessibility was selectively detected in downstream regions of affected genes.

Conclusions:

  • Computational integration of multi-omics data reveals novel mechanisms in lytic HSV-1 infection.
  • Disruption of transcription termination and its impact on chromatin architecture are key findings.
  • These insights advance our understanding of host-virus interactions at a molecular level.