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Fats as Energy Storage Molecules01:06

Fats as Energy Storage Molecules

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Triglycerides are a form of long-term energy storage molecules. They are made of glycerol and three fatty acids. To obtain energy from fat, triglycerides must first be broken down by hydrolysis into their two principal components, fatty acids and glycerol. This process, called lipolysis, takes place in the cytoplasm. The resulting fatty acids are oxidized by β-oxidation into acetyl-CoA, which is used by the Krebs cycle. The glycerol that is released from triglycerides after lipolysis...
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Related Experiment Video

Updated: Aug 16, 2025

Cerenkov Luminescence Imaging of Interscapular Brown Adipose Tissue
06:28

Cerenkov Luminescence Imaging of Interscapular Brown Adipose Tissue

Published on: October 7, 2014

13.4K

Keeping brown fat brown.

Amy E Baek1

  • 1Science Signaling, AAAS, Washington, DC 20005, USA.

Science Signaling
|December 20, 2022
PubMed
Summary

An ER-lipid droplet anchoring protein determines the multilocular phenotype of brown adipose tissue. This protein is crucial for the development and function of brown fat cells.

Area of Science:

  • Cell Biology
  • Metabolism
  • Adipose Tissue Biology

Background:

  • Brown adipose tissue (BAT) plays a key role in thermogenesis and energy expenditure.
  • The multilocular appearance of BAT, characterized by numerous small lipid droplets, is essential for its function.
  • The molecular mechanisms regulating BAT morphology and function are not fully understood.

Purpose of the Study:

  • To identify the protein responsible for anchoring lipid droplets to the endoplasmic reticulum (ER) in brown adipose tissue.
  • To elucidate the role of this anchoring protein in determining the multilocular phenotype of BAT.

Main Methods:

  • Immunofluorescence microscopy to visualize ER-lipid droplet interactions.
  • Lipid droplet isolation and biochemical analysis.

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Visualization and Quantification of Brown and Beige Adipose Tissues in Mice using [18F]FDG Micro-PET/MR Imaging

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Last Updated: Aug 16, 2025

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  • Genetic manipulation to study the function of the identified protein.

    • Main Results:

    • A novel ER-lipid droplet anchoring protein was identified.
    • This protein was found to be essential for the formation of small, numerous lipid droplets in BAT.
    • Knockdown of the protein resulted in a unilocular phenotype and impaired thermogenic capacity.

    Conclusions:

    • The identified ER-lipid droplet anchoring protein is a key determinant of the multilocular phenotype in brown adipose tissue.
    • This protein is critical for BAT function and energy expenditure.
    • Targeting this protein may offer therapeutic strategies for metabolic disorders.