Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

57.7K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
57.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Empirical validation of a generative AI framework for personalized education assessment.

Scientific reports·2026
Same author

Cross-domain Fisher criterion for remote therapeutic efficacy assessment of Parkinson's disease via speech recognition.

Computer methods in biomechanics and biomedical engineering·2025
Same author

Immuno-RAFT Fluorescence Array Sensing for High-Throughput Analysis of CD81 Protein.

Analytical chemistry·2025
Same author

Multi-source sparse broad transfer learning for parkinson's disease diagnosis via speech.

Medical & biological engineering & computing·2025
Same author

In vitro study of the pro-apoptotic mechanism of amino acid Schiff base copper complexes on anaplastic thyroid cancer.

European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences·2025
Same author

Reversible addition-fragmentation chain transfer enhanced aggregation signal-on fluorescence detection of alkaline phosphatase.

Analytical and bioanalytical chemistry·2024

Related Experiment Video

Updated: Aug 16, 2025

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
10:28

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

33.3K

Ferritin-Enhanced Direct MicroRNA Detection via Controlled Radical Polymerization.

Nan Ma1, Yu Zhao1, Lianzhi Li2

  • 1School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, Jiangsu210094, P. R. China.

Analytical Chemistry
|December 21, 2022
PubMed
Summary

This study presents a novel ferritin-enhanced biosensor for ultrasensitive detection of microRNA-21, an early lung cancer marker. The method offers a low-cost, direct gene detection approach for improved cancer diagnostics.

More Related Videos

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
07:27

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

20.7K
Detection of microRNA Expression in Peritoneal Membrane of Rats Using Quantitative Real-time PCR
08:56

Detection of microRNA Expression in Peritoneal Membrane of Rats Using Quantitative Real-time PCR

Published on: June 27, 2017

8.0K

Related Experiment Videos

Last Updated: Aug 16, 2025

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
10:28

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

33.3K
High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
07:27

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

20.7K
Detection of microRNA Expression in Peritoneal Membrane of Rats Using Quantitative Real-time PCR
08:56

Detection of microRNA Expression in Peritoneal Membrane of Rats Using Quantitative Real-time PCR

Published on: June 27, 2017

8.0K

Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Molecular Diagnostics

Background:

  • Accurate quantitative detection of nucleic acids is critical for cancer genetic testing.
  • Developing low-cost, highly sensitive direct gene detection methods is essential for cancer prevention and treatment.
  • microRNA-21 is a known early biomarker for lung cancer.

Purpose of the Study:

  • To develop an ultrasensitive electrochemical biosensor for the direct detection of microRNA-21.
  • To utilize a ferritin-enhanced atom-transfer radical polymerization (Ft-ATRP) process for signal amplification.
  • To establish a cost-effective and highly sensitive method for early lung cancer detection.

Main Methods:

  • Fabrication of an electrochemical biosensor using peptide nucleic acid probes on a gold electrode.
  • Employing ferritin to mediate the aggregation of hydrophilic nitroxide radical monomers (MATMP).
  • Utilizing Ft-ATRP to form polymer chains with electrochemical signals on the target microRNA-21 for signal amplification.

Main Results:

  • The biosensor achieved an ultrasensitive limit of detection (LOD) for microRNA-21 as low as 6.03 fM.
  • The detection concentration range was established from 0.01 to 100 pM with high linearity (R² = 0.994).
  • The developed RNA biosensor demonstrated robust performance in analyzing complex samples with simple operation.

Conclusions:

  • The ferritin-enhanced biosensor provides a highly sensitive and direct method for microRNA-21 detection.
  • This innovative approach shows significant potential for early lung cancer screening and identification.
  • The biosensor's components and detection process exhibit good water solubility, facilitating practical application.