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A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
Published on: December 25, 2015
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Six complex microbial inoculants for removing ammonia nitrogen from waters
Chunmeng Sun1, Cong Li1, Kai Zhang1
1School of Environment and Architecture, University of Shanghai for Science and Technology, Shanghai, China.
Summary
Six microbial inoculants were evaluated for ammonia nitrogen (NH4+-N) removal. Gandu nitrifying bacteria (NR4) showed the best NH4+-N removal, while Bilaiqing (NR5) excelled at reducing chemical oxygen demand (COD).
Area of Science:
- Environmental Microbiology
- Water Quality Management
- Bioremediation
Background:
- Ammonia nitrogen (NH4+-N) is a key pollutant in water bodies, impacting aquatic ecosystems and human health.
- Microbial inoculants offer a promising biological approach for removing NH4+-N from contaminated waters.
- Understanding the microbial community composition is crucial for optimizing bioremediation strategies.
Purpose of the Study:
- To compare the efficacy of six different complex microbial inoculants in removing ammonia nitrogen (NH4+-N).
- To analyze the microbial community composition of these inoculants using high-throughput sequencing.
- To evaluate the impact of these inoculants on chemical oxygen demand (COD) removal.
Main Methods:
- Six distinct complex microbial inoculants were subjected to laboratory testing for NH4+-N and COD removal.
- High-throughput sequencing was employed to determine the microbial community structure before and after treatment.
- Single-factor experiments were conducted to identify optimal conditions for the most effective inoculant.
Main Results:
- Proteobacteria and Firmicutes were identified as the dominant phyla across the inoculant samples.
- Gandu nitrifying bacteria (NR4) demonstrated the highest NH4+-N removal efficiency.
- Bilaiqing ammonia nitrogen removal bacteria agent (NR5) exhibited the best performance in reducing COD.
- Optimal conditions for NR4 were determined as pH 7, 30°C, 1.0 g/L dosage, and NH4+-N concentrations from 30 to 300 mg/L.
Conclusions:
- Specific microbial inoculants, such as Gandu nitrifying bacteria (NR4), are highly effective for ammonia nitrogen removal in aquatic environments.
- Microbial community analysis provides valuable insights into the mechanisms of NH4+-N removal, though relative abundance does not directly correlate with removal ability.
- These findings support the application of selected microbial agents for improving water quality in urban landscape water bodies.
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