Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chemotaxis and Direction of Cell Migration01:21

Chemotaxis and Direction of Cell Migration

3.4K
Cells can detect chemical cues in their environment and reorganize the cytoskeleton to migrate toward them or away from them. This directional migration, called chemotaxis, is essential during embryogenesis and development, immune response, tissue repair and regeneration, and reproduction. These chemical cues can either attract or repel the cell's movement. For example, axon development is determined by a combination of chemoattractants and chemorepellents that direct the growing axon...
3.4K
Cancer Cell Migration through Invadopodia01:35

Cancer Cell Migration through Invadopodia

2.4K
Invadosome is a broad category of cell surface structures with proteolytic activity that  degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However,...
2.4K
Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

4.8K
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
4.8K
Cell Migration01:09

Cell Migration

17.1K
Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
17.1K
Selectins01:25

Selectins

3.4K
Cell adhesion is  an essential aspect of multicellularity. While stable cell interactions usually occur between cells of the same type, transient cell interactions occur between cells of different tissue types, such as between neutrophils and endothelial cells. Selectins are one class of cell adhesion molecules (CAMs) that bind carbohydrate ligands to form transient cell adhesion. They are rod-like proteins with a long extracellular part of variable length ending with the lectin domain,...
3.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Advancements in extracellular vesicle research.

Extracellular vesicle·2026
Same author

15-LOX-catalytic bias towards ether-(alkenyl)-ETE-PEs oxidation bestows selectivity of PRO-ferroptotic cell death signaling.

Nature communications·2026
Same author

Harnessing myeloid cell plasticity for cancer therapy.

Nature cancer·2026
Same author

Mitochondrial double-stranded RNA fuels pancreatic cancer growth via RIG-I/TLR3 inflammation.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

The origin of hepatocellular carcinoma depends on metabolic zonation.

Science (New York, N.Y.)·2025
Same author

Parkin Induces Ubiquitination and Large Extracellular Vesicle Release of HMGB1 to Activate Antitumor Immunity.

Cancer research·2025

Related Experiment Video

Updated: Aug 16, 2025

Bioparticle Microarrays for Chemotactic and Molecular Analysis of Human Neutrophil Swarming in vitro
11:21

Bioparticle Microarrays for Chemotactic and Molecular Analysis of Human Neutrophil Swarming in vitro

Published on: February 16, 2020

5.2K

Syntaphilin Regulates Neutrophil Migration in Cancer.

Shuyu Fu1, Hui Deng1, Irene Bertolini1

  • 1Immunology, Microenvironment and Metastasis Program, Wistar Institute, Philadelphia, Pennsylvania.

Cancer Immunology Research
|December 22, 2022
PubMed
Summary

Syntaphilin (SNPH) regulates neutrophil migration in cancer. Decreased SNPH increases myeloid-derived suppressor cell migration, promoting tumor metastasis and suggesting new therapeutic targets.

More Related Videos

Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents
14:04

Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents

Published on: March 25, 2016

15.4K
Microfluidic Platform for Measuring Neutrophil Chemotaxis from Unprocessed Whole Blood
10:13

Microfluidic Platform for Measuring Neutrophil Chemotaxis from Unprocessed Whole Blood

Published on: June 3, 2014

11.4K

Related Experiment Videos

Last Updated: Aug 16, 2025

Bioparticle Microarrays for Chemotactic and Molecular Analysis of Human Neutrophil Swarming in vitro
11:21

Bioparticle Microarrays for Chemotactic and Molecular Analysis of Human Neutrophil Swarming in vitro

Published on: February 16, 2020

5.2K
Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents
14:04

Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents

Published on: March 25, 2016

15.4K
Microfluidic Platform for Measuring Neutrophil Chemotaxis from Unprocessed Whole Blood
10:13

Microfluidic Platform for Measuring Neutrophil Chemotaxis from Unprocessed Whole Blood

Published on: June 3, 2014

11.4K

Area of Science:

  • Immunology
  • Cancer Biology
  • Cell Biology

Background:

  • Pathologically activated neutrophils, termed myeloid-derived suppressor cells (PMN-MDSC), promote tumor progression and metastasis.
  • PMN-MDSC facilitate premetastatic niche formation through enhanced migration from bone marrow.
  • The molecular mechanisms controlling PMN migration in cancer remain largely unknown.

Purpose of the Study:

  • To investigate the role of syntaphilin (SNPH) in regulating neutrophil migration.
  • To elucidate the molecular mechanisms by which SNPH influences PMN migration and metastasis.

Main Methods:

  • Analysis of SNPH expression in neutrophils from tumor-bearing and healthy individuals/mice.
  • Assessment of PMN migration, metastasis, mitochondrial dynamics, and metabolic activity in syntaphilin-knockout (SNPH-KO) mice.
  • Measurement of adenosine generation in PMN.

Main Results:

  • SNPH expression is decreased in PMN from tumor-bearing mice and cancer patients.
  • SNPH-KO mice exhibit increased spontaneous PMN migration and enhanced tumor metastasis.
  • In SNPH-KO mice, PMN show increased mitochondrial transport, elevated oxidative phosphorylation and glycolysis, and heightened adenosine production.

Conclusions:

  • Syntaphilin (SNPH) is a key regulator of PMN migration.
  • Reduced SNPH expression contributes to increased PMN migration and metastasis in cancer.
  • Targeting SNPH presents a potential therapeutic strategy for inhibiting cancer progression.