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Updated: Aug 16, 2025
Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation
Published on: August 21, 2019
CyanoMapDB: a database integrating experimentally validated protein-protein interactions in cyanobacteria
Zhao Peng1, Jiaqiang Li2, Xingpeng Jiang2
1School of Life Sciences, and Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University, Wuhan 430079, Hubei, People's Republic of China.
Researchers developed CyanoMapDB, a new database for cyanobacterial protein-protein interactions (PPIs). This resource integrates experimental data, aiding the study of these essential microorganisms and their functions.
Area of Science:
- Microbiology
- Biochemistry
- Bioinformatics
Background:
- Cyanobacteria are vital biosphere organisms with growing research interest.
- Protein-protein interactions (PPIs) are crucial for biological functions but are understudied in cyanobacteria.
- Existing PPI data for cyanobacteria is fragmented and not user-friendly.
Purpose of the Study:
- To create a comprehensive, integrated database of cyanobacterial PPIs.
- To provide easy access to experimentally validated PPI data for cyanobacteria.
- To facilitate research on cyanobacterial biology and plant science.
Main Methods:
- Integrated PPI data from UniProt, STRING, and IntAct.
- Mined additional PPIs from co-fractionation Mass Spectrometry (MS) data in cyanobacteria.
- Developed the CyanoMapDB web resource (http://www.cyanomapdb.msbio.pro/) for data accessibility.
Main Results:
- CyanoMapDB contains 52,304 PPIs involving 6,789 proteins from 23 cyanobacterial species.
- The database offers integrated, experimentally evidenced PPI data.
- Users can query proteins, view interacting partners, and visualize interaction networks.
Conclusions:
- CyanoMapDB provides a valuable, integrated resource for cyanobacterial PPIs.
- The database simplifies access to complex interaction data.
- CyanoMapDB is expected to significantly advance research in cyanobacteria and related plant studies.
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02:04Protein-protein Interfaces
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