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Gut microbiota profiling variated during colorectal cancer development in mouse
Jingjing Liu1,2, Wei Dong1, Jian Zhao1
1Department of Biomedical Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing, 210016, China.
BMC Genomics
|December 22, 2022
Summary
Changes in gut bacteria are linked to colorectal cancer development. This study found significant shifts in intestinal flora, with a decrease in Muribaculaceae and an increase in Bacteroidaceae, suggesting their role in colorectal cancer progression.
Area of Science:
- Microbiology
- Oncology
- Gastroenterology
Background:
- Intestinal flora imbalance is implicated in colorectal cancer (CRC) development.
- Understanding gut microbiota changes during CRC progression is crucial for mechanistic insights.
Purpose of the Study:
- To investigate alterations in the gut microbiota during colorectal inflammation and cancer.
- To identify specific bacterial taxa and functional pathways associated with CRC development.
Main Methods:
- Deep sequencing of the 16S ribosomal RNA gene in fecal samples from mice.
- Analysis of microbial community structure using PCoA and ANOSIM tests.
- Correlation analysis to identify interactions between bacterial families and functional pathway analysis.
Main Results:
- Significant changes in intestinal flora were observed in mice with colorectal inflammation and CRC compared to healthy controls (p < 0.05).
- Muribaculaceae and Bacteroidaceae exhibited strong reciprocal exclusion patterns.
- Functional analysis revealed upregulation of cancer pathways and downregulation of replication and repair pathways in the gut microbiota.
Conclusions:
- The gut microbiota composition is significantly altered in mice with colorectal inflammation and CRC.
- A decrease in Muribaculaceae and an increase in Bacteroidaceae were specifically noted.
- These shifts suggest a potential role for Muribaculaceae and Bacteroidaceae in the pathogenesis of colorectal cancer.
