Effects of Calcium Ions on the Antimicrobial Activity of Gramicidin A

Gramicidin A (gA) is a linear antimicrobial peptide that can form a channel and specifically conduct monovalent cations such as H⁺ across the lipid membrane. The antimicrobial activity of gA is associated with the formation of hydroxyl free radicals and the imbalance of NADH metabolism, possibly a consequence caused by the conductance of cations. The ion conductivity of gramicidin A can be blocked by Ca²⁺ ions. However, the effect of Ca²⁺ ions on the antimicrobial activity of gA is unclear. To unveil the role of Ca²⁺ ions, we examined the effect of Ca²⁺ ions on the antimicrobial activity of gramicidin A against Staphylococcus aureus (S. aureus). Results showed that the antimicrobial mechanism of gA and antimicrobial activity by Ca²⁺ ions are concentration-dependent. At the low gA concentration (≤1 μM), the antimicrobial mechanism of gA is mainly associated with the hydroxyl free radical formation and NADH metabolic imbalance. Under this mode, Ca²⁺ ions can significantly inhibit the hydroxyl free radical formation and NADH metabolic imbalance. On the other hand, at high gA concentration (≥5 μM), gramicidin A acts more likely as a detergent. Gramicidin A not only causes an increase in hydroxyl free radical levels and NAD⁺/NADH ratios but also induces the destruction of the lipid membrane composition. At this condition, Ca²⁺ ions can no longer reduce the gA antimicrobial activity but rather enhance the bacterial killing ability of gramicidin A.