Quantifying Antibiotic Distribution in Solid and Liquid Fractions of Manure Using a Two-Step, Multi-Residue
Carlton Poindexter1, Andrea Yarberry1,2, Clifford Rice2
1Department of Environmental Science and Technology, University of Maryland, College Park, MD 20742, USA.
Antibiotics (Basel, Switzerland)
|December 23, 2022
Summary
A new method uniformly extracts four antibiotic classes from liquid and solid manure. This improves antibiotic analysis in manure, aiding risk assessment for agricultural applications.
Area of Science:
- Environmental Chemistry
- Analytical Chemistry
- Agricultural Science
Background:
- Antibiotic distribution in manure is variable, complicating analysis.
- Understanding antibiotic presence in manure is crucial for environmental risk assessment.
Purpose of the Study:
- To develop and validate a single extraction method for multiple antibiotic classes in diverse manure matrices.
- To enable precise risk assessment of antibiotic transport in manure waste streams.
Main Methods:
- Developed a two-step liquid-solid extraction protocol using EDTA-McIlvaine buffer and methanol.
- Applied the method to unprocessed manure, solid-liquid separated manure, and composted solids.
- Analyzed 10 antibiotics across tetracycline, sulfonamide, macrolide, and β-lactam classes using liquid chromatography tandem mass spectrometry.
Main Results:
- Achieved relative standard deviations < 30% for most antibiotics across varied manure substrates.
- Reported total antibiotic recoveries: 67–131% for tetracyclines, 56% for sulfonamides, 49–53% for macrolides, and 1.3–66% for β-lactams.
- Demonstrated the method's effectiveness for simultaneous analysis of four antibiotic classes in liquid and solid manure fractions.
Conclusions:
- This study presents the first unified protocol for assessing multiple antibiotic classes in different manure forms.
- The developed method enhances the accuracy of antibiotic analysis in manure, supporting better environmental risk management.
Related Concept Videos
01:30Measurement of Bioavailability: Pharmacokinetic Methods
Pharmacokinetics is a vital branch of pharmacology that examines how drugs are absorbed, distributed, metabolized, and excreted by the body. Two key methodologies in pharmacokinetics are plasma drug concentration studies and urinary drug excretion analyses, both of which provide critical insights into a drug's therapeutic efficacy and bioavailability.Plasma Drug Concentration-Time StudiesPlasma drug concentration-time studies involve analyzing blood samples at specific intervals to quantify...
01:15Determination of Multiple Dosing Parameters: Steady-State, Minimum and Maximum Concentrations
Gentamicin, an aminoglycoside antibiotic, is commonly administered via intermittent intravenous infusion to treat severe infections. An intermittent one-hour infusion of gentamicin, administered at eight-hour intervals, allows for precise control of plasma drug concentrations, minimizing toxicity while ensuring therapeutic efficacy. Pharmacokinetic principles govern the dynamics of plasma concentrations and can be mathematically described using specific equations.The plasma drug concentration...