Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tree-Based Machine Learning Model for Fluorescence Lifetime Prediction in Organic Compounds.

Journal of fluorescence·2026
Same author

In vivo resonance micro-Raman spectroscopy study of Artemia salina (Linnaeus, 1758) carotenoids profile as proxy for variability in hypersaline lakes environmental conditions.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2026
Same author

Comparative and Optimized Chemical Synthesis of AgNPs for Improved Surface Reactivity and Potential Biosensing Applications.

Nanomaterials (Basel, Switzerland)·2025
Same author

Aged Biogenic Carbonates from Crustacean Waste: Structural and Functional Evaluation of Calibrated Fine Powders and Their Conversion into Phosphate Minerals.

Materials (Basel, Switzerland)·2025
Same author

SERS Detection of Environmental Variability in Balneary Salt Lakes During Tourist Season: A Pilot Study.

Biosensors·2025
Same author

Pharmaceutical Co-crystal of Ketoconazole-adipic Acid: Excipient Compatibility and In Silico Antifungal Potential Studies.

Pharmaceutical research·2025

Related Experiment Video

Updated: Aug 16, 2025

Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging
06:19

Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging

Published on: June 9, 2023

1.6K

Detection and Characterization of Nodularin by Using Label-Free Surface-Enhanced Spectroscopic Techniques.

Ioana Andreea Brezeștean1,2, Ana Maria Raluca Gherman1, Alia Colniță1

  • 1Department of Molecular and Biomolecular Physics, National Institute for Research and Development of Isotopic and Molecular Technologies, 400293 Cluj-Napoca, Romania.

International Journal of Molecular Sciences
|December 23, 2022
PubMed
Summary

Nodularin (NOD), a cyanobacteria toxin, can be detected using Raman spectroscopy. This study details a new method for identifying NOD, even with other toxins present, achieving a low detection limit.

Keywords:
RamanSERScyanotoxinmicrocystinsnodularin

More Related Videos

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

20.4K
A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
09:09

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

8.7K

Related Experiment Videos

Last Updated: Aug 16, 2025

Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging
06:19

Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging

Published on: June 9, 2023

1.6K
Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

20.4K
A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
09:09

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

8.7K

Area of Science:

  • Environmental Science
  • Analytical Chemistry
  • Spectroscopy

Background:

  • Nodularin (NOD) is a potent toxin produced by *Nodularia spumigena* cyanobacteria.
  • NOD often co-exists with microcystins in environmental waters, complicating toxin identification.
  • Accurate detection methods are crucial for monitoring water safety.

Purpose of the Study:

  • To perform a complete theoretical DFT-vibrational Raman characterization of Nodularin (NOD).
  • To investigate the utility of various Surface-Enhanced Raman Spectroscopy (SERS) substrates for NOD detection.
  • To establish a sensitive and specific method for NOD identification in environmental samples.

Main Methods:

  • Density Functional Theory (DFT) calculations for theoretical Raman spectra.
  • Experimental Drop-Coating Deposition Raman (DCDR) technique.
  • SERS analysis using silver nanoparticles (AgNPs), Klarite™ substrate, Tienta® SpecTrim™ slides, and nanoimprint lithography (NIL) fabricated nanotrenches.
  • Utilized a 532 nm excitation source for optimal SERS performance.

Main Results:

  • Comprehensive theoretical vibrational Raman characterization of NOD was achieved.
  • SERS analysis demonstrated the effectiveness of Klarite™ substrate and AgNPs for NOD detection.
  • A low limit of detection (LOD) of 8.4 × 10⁻⁸ M for NOD in solution was obtained using AgNPs.
  • The 532 nm laser provided well-defined bands and high SERS intensity, especially at low concentrations.

Conclusions:

  • Theoretical DFT-vibrational Raman analysis provides unambiguous assignment of NOD spectral bands.
  • SERS, particularly with AgNPs and Klarite™ substrates, offers a sensitive method for NOD detection.
  • This approach is promising for identifying NOD in complex environmental matrices, aiding water quality monitoring.