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New Therapeutics for Extracellular Vesicles: Delivering CRISPR for Cancer Treatment

Biying Yan1, Yaxuan Liang1

  • 1Center for Biological Science and Technology, Advanced Institute of Natural Sciences, Beijing Normal University, Zhuhai 519087, China.

International Journal of Molecular Sciences
|December 23, 2022
PubMed

Insights

Extracellular vesicles (EVs) show promise as natural delivery systems for CRISPR gene editing in cancer therapy. This review highlights EV-mediated CRISPR strategies for various cancers, addressing delivery challenges and clinical potential.

Area of Science:

  • Biotechnology
  • Genetics
  • Oncology

Background:

  • Cancers originate from genetic defects, making gene therapy a promising treatment avenue.
  • CRISPR technology offers precise gene editing capabilities but faces delivery vector limitations for clinical use.

Purpose of the Study:

  • To review the biology and function of CRISPR systems.
  • To summarize current CRISPR delivery methods, focusing on extracellular vesicles (EVs).
  • To highlight the clinical potential of EV-mediated CRISPR for cancer treatment.

Main Methods:

  • Review of current literature on CRISPR technology and gene delivery vectors.
  • Emphasis on extracellular vesicles (EVs) as natural nanocarriers for CRISPR components.
  • Analysis of strategies for constructing EV-CRISPR vectors for cancer applications.

Main Results:

  • Extracellular vesicles (EVs) are emerging as effective and superior vectors for delivering CRISPR gene editing tools.
  • EV-mediated CRISPR editing has shown progress in targeting various cancer types and specific genes.
  • Current strategies for engineering EV-CRISPR vectors and their limitations are discussed.

Conclusions:

  • Engineered EVs hold significant clinical potential as delivery systems for CRISPR-based cancer gene therapy.
  • Further development of EV-CRISPR technology is crucial for expanding the cancer treatment toolkit.
  • Overcoming delivery challenges is key to translating EV-CRISPR therapies into clinical practice.
Keywords:
CRISPRCas9cancerexosomeextracellular vesiclesgene editinggene therapy

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