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Gamma Irradiation Processing on 3D PCL Devices-A Preliminary Biocompatibility Assessment.
Fernando Guedes1,2,3, Mariana V Branquinho1,2,3, Sara Biscaia4
1Veterinary Clinics Department, Abel Salazar Biomedical Sciences Institute (ICBAS), University of Porto (UP), Rua de Jorge Viterbo Ferreira, n° 228, 4050-313 Porto, Portugal.
International Journal of Molecular Sciences
|December 23, 2022
Summary
Gamma radiation effectively sterilizes polycaprolactone (PCL) scaffolds for medical devices. Doses of 35 kGy and 45 kGy demonstrated superior cytocompatibility, making them ideal for tissue engineering applications.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Medical Device Sterilization
Background:
- Polycaprolactone (PCL)-based scaffolds are crucial in tissue engineering for medical devices.
- Sterilization is a critical step for safe in vitro and in vivo application of PCL scaffolds.
- Gamma radiation is a common sterilization method, but its effect on PCL properties needs careful evaluation.
Purpose of the Study:
- To determine the optimal gamma radiation sterilization conditions for PCL-based scaffolds.
- To assess the biocompatibility and cytocompatibility of PCL scaffolds after gamma irradiation.
- To inform future applications of PCL scaffolds in treating airway obstruction.
Main Methods:
- PCL-based scaffolds were sterilized using three gamma radiation doses: 25 kGy, 35 kGy, and 45 kGy.
- Biocompatibility and cytocompatibility of the sterilized scaffolds were evaluated.
- The study focused on preliminary assessments for potential use in airway obstruction conditions.
Main Results:
- All tested gamma radiation levels (25, 35, and 45 kGy) resulted in biocompatible PCL scaffolds.
- Scaffolds sterilized with 35 kGy and 45 kGy exhibited enhanced cytocompatibility compared to 25 kGy.
- The 45 kGy sterilization dose showed the most favorable overall outcomes in terms of cytocompatibility.
Conclusions:
- Gamma radiation sterilization is suitable for PCL-based scaffolds.
- A dose of 45 kGy is recommended for sterilizing PCL scaffolds intended for medical applications, particularly in tissue engineering for airway conditions.
- These findings support the use of 3D-printed PCL scaffolds for treating tracheal lesions.
Keywords:
3D printingbiocompatibilitycytocompatibilitygamma irradiationmesenchymal stem cellspolycaprolactone
