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Related Experiment Video

Updated: Aug 16, 2025

Combining Human Organoids and Organ-on-a-Chip Technology to Model Intestinal Region-Specific Functionality
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Combining Human Organoids and Organ-on-a-Chip Technology to Model Intestinal Region-Specific Functionality

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Microfluidic Device to Manipulate 3D Human Epithelial Cell-Derived Intestinal Organoids.

Miki Matsumoto1, Yuya Morimoto2, Toshiro Sato3

  • 1Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.

Micromachines
|December 23, 2022
PubMed
Summary

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This summary is machine-generated.

Researchers developed a microfluidic organoid-trapping device to immobilize human intestinal organoids for fluidic stimulation. This novel device enables organoid culture and fusion, advancing research in organoid behavior under controlled conditions.

Area of Science:

  • Biomedical Engineering
  • Microfluidics
  • Organoid Technology

Background:

  • Human intestinal organoids are valuable models for studying gut biology and disease.
  • Current methods for applying controlled stimuli to organoids are limited.
  • Immobilizing organoids without causing deformation is crucial for accurate experimentation.

Purpose of the Study:

  • To develop and validate a microfluidic device for immobilizing and stimulating human intestinal organoids.
  • To demonstrate the device's capability for culturing organoids and inducing fusion.
  • To explore the potential of fluidic shear stress in regulating organoid surface structures.

Main Methods:

  • Design of a microfluidic device featuring a trapping region with wall gaps and a constriction.
Keywords:
enteroidfusionintestinal organoidmicrofluidicsperfusion

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  • Utilizing hydrostatic pressure differences to culture organoids without deformation.
  • Applying fluidic shear stress to aligned organoids to induce fusion and modify surface structures.

    • Main Results:

    • Successful immobilization of human intestinal organoids within the microfluidic device.
    • Demonstrated culturing of organoids without significant deformation.
    • Achieved organoid fusion and regulation of surface structures via controlled fluidic stimuli.

    Conclusions:

    • The microfluidic organoid-trapping device is effective for immobilizing and culturing human intestinal organoids.
    • The device facilitates controlled application of fluidic stimuli, enabling organoid fusion and surface structure modulation.
    • This technology holds promise for advancing research in organoid-based studies and drug screening.