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Related Concept Videos

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Related Experiment Video

Updated: Aug 16, 2025

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography
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High-aperture EUV microscope using multilayer mirrors and a 3D reconstruction algorithm based on z-tomography.

I V Malyshev, D G Reunov, N I Chkhalo

    Optics Express
    |December 23, 2022
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    Summary
    This summary is machine-generated.

    This study introduces a novel EUV microscope for 3D imaging of biological samples. It achieves high resolution and enables z-tomography for unprecedented cellular visualization.

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    Area of Science:

    • Optics and Photonics
    • Biophysics
    • Microscopy

    Background:

    • Advancements in microscopy are crucial for understanding biological structures.
    • Existing techniques face limitations in 3D resolution and sample penetration.
    • Extreme Ultraviolet (EUV) microscopy offers potential for high-resolution imaging.

    Purpose of the Study:

    • To develop an EUV microscope for high-resolution 3D imaging of bio-samples.
    • To demonstrate the feasibility of z-tomography with EUV microscopy.
    • To introduce a new algorithm for 3D image reconstruction from tomographic data.

    Main Methods:

    • Development of an EUV microscope operating at 13.84 nm wavelength.
    • Utilized a mirror lens with a numerical aperture (NA) of 0.27.
    • Implemented z-tomography and a novel 3D reconstruction algorithm accounting for aberrations and point spread function.

    Main Results:

    • Achieved a pixel resolution of 140 nm for reconstructed 3D images.
    • Successfully performed z-tomography on bio-samples for the first time with this EUV microscope.
    • The new algorithm reconstructed absorption images with a 10% error for cells up to 10 µm thick.

    Conclusions:

    • The developed EUV microscope enables high-resolution 3D imaging and z-tomography of biological specimens.
    • The novel reconstruction algorithm is effective for absorption imaging, reducing complexity and error.
    • This technology opens new avenues for studying cellular structures in three dimensions.