Related Experiment Video
Updated: Aug 16, 2025
Generation of Prostate Cancer Cell Models of Resistance to the Anti-mitotic Agent Docetaxel
Published on: September 8, 2017
Bioresorbable Nonwoven Patches as Taxane Delivery Systems for Prostate Cancer Treatment
Joanna Jaworska1, Arkadiusz Orchel2, Anna Kaps2
1Centre of Polymer and Carbon Materials, Polish Academy of Sciences, M. Curie-Sklodowskiej 34, 41-819 Zabrze, Poland.
Abstract:
Prostate cancer is the second most common cancer in males. In the case of locally advanced prostate cancer radical prostatectomy is one of the first-line therapy. However, recurrence after resection of the tumor can appear. Drug-eluting bioresorbable implants acting locally in the area of the tumor or the resection margins, that reduce the risk of recurrence would be advantageous. Electrospinning offers many benefits in terms of local delivery so fiber-forming polyesters and polyestercarbonates which are suitable to be drug-loaded were used in the study to obtain CTX or DTX-loaded electrospun patches for local delivery. After a fast verification step, patches based on the blend of poly(glycolide-ε-caprolactone) and poly(lactide-glycolide) as well as patches obtained with poly(lactide-glycolide- ε-caprolactone) were chosen for long-term study. After three months, 60% of the drug was released from (PGCL/PLGA) + CTX and it was selected for final, anticancer activity analysis with the use of PC-3 and DU145 cells to establish its therapeutic potential. CTX-loaded patches reduced cell growth to 53% and 31% respectively, as compared to drug-free patches. Extracts from drug-free patches showed excellent biocompatibility with the PC-3 cell line. Cabazitaxel-loaded bioresorbable patches are a promising drug delivery system for prostate cancer therapy.
Insights
Drug-eluting bioresorbable patches loaded with Cabazitaxel (CTX) show promise for treating prostate cancer. These patches effectively reduce cancer cell growth locally after surgery, offering a potential new therapy.
Area of Science:
- Biomaterials Science
- Oncology
- Drug Delivery Systems
Background:
- Prostate cancer is a leading cancer in males, often treated with radical prostatectomy.
- Recurrence post-surgery is a significant clinical challenge.
- Local drug delivery systems could reduce recurrence risk at tumor sites or resection margins.
Purpose of the Study:
- To develop and evaluate drug-eluting bioresorbable electrospun patches for local delivery of chemotherapy agents.
- To assess the drug release profile, anticancer activity, and biocompatibility of these patches for prostate cancer therapy.
Main Methods:
- Electrospinning was used to create drug-loaded patches using polyesters and polyestercarbonates.
- Patches loaded with Cabazitaxel (CTX) or Docetaxel (DTX) were fabricated.
- In vitro drug release, cytotoxicity assays using PC-3 and DU145 cells, and biocompatibility tests were performed.
Main Results:
- Cabazitaxel (CTX)-loaded patches demonstrated sustained drug release over three months (60% release).
- CTX-loaded patches significantly reduced prostate cancer cell growth (53% for PC-3, 31% for DU145) compared to controls.
- Drug-free patches exhibited excellent biocompatibility with the PC-3 cell line.
Conclusions:
- Cabazitaxel-loaded bioresorbable electrospun patches represent a promising local drug delivery system for prostate cancer.
- This approach has therapeutic potential for reducing post-surgical recurrence.
- The developed patches show good biocompatibility and efficacy in preclinical models.
