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Programmatically Dynamic Microcompartmentation in Coacervate-in-Pickering Emulsion Protocell.

Mengqing Chen1, Guoliang Liu1, Ming Zhang2

  • 1College of Chemistry and Molecule Sciences, Wuhan University, Wuhan, 430072, China.

Small (Weinheim an Der Bergstrasse, Germany)
|December 23, 2022
PubMed
Summary
This summary is machine-generated.

This study introduces a stable coacervate-in-Pickering emulsion protocell model for artificial cell development. This system offers dynamic microcompartmentation and stability, crucial for advancing cellular biology and creating lifelike systems.

Keywords:
Pickering emulsioncoacervatemulti-compartmentsprogrammableprotocells

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Area of Science:

  • Synthetic Biology
  • Biophysics
  • Materials Science

Background:

  • Advancing artificial cell research requires methods for complex cellular mechanisms.
  • Creating synthetic cells with dynamic multi-compartments and stable membranes remains a significant challenge.

Purpose of the Study:

  • To develop a stable, dynamic protocell model using a coacervate-in-Pickering emulsion.
  • To demonstrate control over intracellular component distribution and enzyme activity.

Main Methods:

  • Fabrication of a coacervate-in-Pickering emulsion protocell via one-step emulsification using poly-l-lysine (PLys) and adenosine triphosphate (ATP).
  • Manipulation of dynamic coacervate distribution using temperature and pH stimuli.
  • Encapsulation of antagonistic enzymes (glucose oxidase and urease) to control local concentrations.

Main Results:

  • The protocell system demonstrated repeatable cycle stability under thermal (24 cycles) and pH (3 cycles) stimuli.
  • Local enzyme concentrations were modulated by introducing glucose and urea, altering pH within the emulsion droplets.
  • The system achieved programmatically dynamic microcompartmentation and sufficient stability.

Conclusions:

  • The developed coacervate-in-Pickering emulsion protocell offers a stable and dynamic platform for artificial cell research.
  • This model facilitates the study of cellular biology and the development of lifelike systems with practical applications.