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Individual Culturing of Tigriopus Copepods and Quantitative Analysis of Their Mate-guarding Behavior

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Embryogenesis of a calanoid copepod analyzed by transcriptomics.

Miguel Cifuentes Acebal¹, Louise Torp Dalgaard¹, Tue Sparholt Jørgensen²

¹Department of Science and Environment, Roskilde University, Universitetsvej 1, DK-4000 Roskilde, Denmark.

Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics

|December 24, 2022

Summary

This study presents the first genome-wide transcriptional map of Acartia tonsa subitaneous embryogenesis. It reveals key developmental gene expression patterns, establishing a framework for future crustacean development research.

Keywords:

Enrichment of pathways Invertebrate genomics Subitaneous embryogenesis, comparative genomics Transcriptome assembly

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Area of Science:

Marine Biology
Developmental Biology
Genomics

Background:

Acartia tonsa is a key model organism and potential live feed for fish larvae.
Previous research focused on adult A. tonsa genomes and transcriptomes.
Genome-wide transcriptional changes during embryogenesis were previously unstudied.

Purpose of the Study:

To investigate genome-wide transcriptional changes during Acartia tonsa subitaneous embryogenesis.
To establish a molecular framework for studying A. tonsa embryonic development.
To enable further use of A. tonsa as a model for crustacean development.

Main Methods:

RNA extraction and de novo transcriptome assembly.
Analysis of gene expression throughout 42-hour embryonic development.
Identification of sequences annotated to known developmental genes.

Main Results:

A comprehensive transcriptome assembly for subitaneous embryogenesis, including quiescent development stages.
Identification of well-known developmental genes.
Detailed description of molecular changes and gene expression levels during embryonic development.

Conclusions:

The study provides the most complete genome-wide transcriptional map of early copepod embryonic development to date.
This map facilitates future research on Acartia tonsa as a model organism.
It lays the groundwork for comparative genomics and pathway enrichment studies in crustaceans.