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Related Concept Videos

Gonadal and Placental Hormones01:24

Gonadal and Placental Hormones

The gonads, namely the testes in males and the ovaries in females, are pivotal in producing gonadal hormones that orchestrate the intricate processes of sexual development and reproduction.
In males, testosterone is the primary gonadal androgen. It plays a central role in the maturation of male reproductive organs — the penis and testes. Additionally, testosterone is instrumental in the development of secondary sexual characteristics — a deep voice as well as facial and pubic hair growth — and...

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A transcriptome dataset for gonadectomy-induced changes in rat spinal cord.

Shawn Miller1, Juan E Abrahante2, Avtar Roopra3

  • 1Divisions of Physical Therapy and Rehabilitation Science, Department of Rehabilitation Medicine, University of Minnesota Medical School, Minneapolis, MN, 55455, USA.

Scientific Data
|December 29, 2022
PubMed
Summary

Gonadectomy significantly alters gene expression in the ventral spinal cord of both male and female rats. This study provides crucial RNA sequencing data on sex steroid hormone effects on motor function circuits.

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Area of Science:

  • Neuroscience
  • Endocrinology
  • Molecular Biology

Background:

  • Sex steroid hormones are vital for central nervous system neural function and neuroplasticity.
  • Spinal cord research on steroid hormones primarily focuses on pain processing in dorsal circuits, with limited understanding of their effects on ventral motor function.
  • Gonadectomy, the removal of testes or ovaries, is a method to study the impact of reduced sex steroids on neural function.

Purpose of the Study:

  • To investigate the impact of gonadectomy on the transcriptome of the ventral spinal cord in adult female and male rats.
  • To provide a next-generation RNA sequencing dataset for analyzing hormonal influences on ventral spinal cord motor circuits.

Main Methods:

  • Adult female and male rats underwent gonadectomy to reduce circulating sex steroid hormones.
  • Next-generation RNA sequencing (RNA-seq) was employed to analyze the transcriptome of ventral spinal cord tissue.

Main Results:

  • Gonadectomy led to significant changes in gene expression within the ventral spinal cord of both sexes.
  • The RNA-seq dataset reveals widespread transcriptomic alterations in response to reduced sex steroid levels.

Conclusions:

  • Gonadectomy profoundly impacts the molecular landscape of the ventral spinal cord.
  • This study offers valuable insights into the role of sex steroid hormones in regulating spinal motor function at a transcriptomic level.